BenchMark™ Pre-Stained Protein Ladder

The BenchMark™ Pre-Stained Protein Ladder is designed for use in SDSPAGE using Tris-Glycine gels for estimation of protein molecular weight, and evaluation of western transfer efficiency. Covalently coupled dyes allows easy visualization of the ladder during, and after electrophoresis.

Features of the BenchMark™ Pre-Stained Protein Ladder:

• Consists of 10 pre-stained protein bands in the range of 6–180 kDa (proteins are covalently coupled to a blue or pink dye)
• Includes a protein coupled to a pink dye (fourth band from the top) to ensure proper identification of each protein band
• Supplied in a ready-to-use format

Note: Coupling of the chromophores to the proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. Use pre-stained protein ladders to determine the approximate molecular weight of proteins. For accurate molecular weight estimation of proteins, use unstained protein ladders.

Specifications

Contents: 2 × 250 μl of BenchMark™ Pre-Stained Protein Ladder
Storage Buffer: 50 mM Tris-HCl, pH 6.8; 5 mM EDTA; 10 mM DTT;
1% (w/v) SDS; 10% (w/v) glycerol
Storage: Store at –20°C
Stability: 1 year at –20°C

Directions

The BenchMark™ Pre-Stained Protein Ladder is supplied in a ready-to-use format.

Do not heat or add reducing agent to the ladder.

1. Thaw the ladder at room temperature.


2. Vortex gently to ensure the solution is homogenous.


3. Load the ladder into the well of an SDS gel using the loading volumes listed below to obtain the best results:

Gel	Electrophoresis	Blotting
Mini-Gel	10 μl	5 μl
Standard Gel	20 μl	10 μl