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Introduction
Protein A Agarose is prepared by covalently coupling purified Protein A to 4% crosslinked agarose beads by a cyanogen bromide method. Protein A Agarose can bind >18 mg of human IgG per mL of gel, a value comparable to that reported by Hjelm et. al (1).
Protein A Agarose binds specifically to the Fc region of IgG molecules. As a result of this property, Protein A Agarose has been used to accomplish the following:
Contents
Protein A Agarose is supplied as a suspension in buffer containing 0.02 M sodium phosphate, (pH 7.2), 0.15 M sodium chloride, 0.02% (w/v) merthiolate.
Intended Use
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Product Qualification
The Certificate of Analysis provides detailed quality control information for each product. Certificates of Analysis are available on our website at www.invitrogen.com/support.
Protein A Agarose binds specifically to the Fc region of IgG molecules. As a result of this property, Protein A Agarose has been used to accomplish the following:
- Purification of IgG (1,2)
- Fractionation of IgG fragments (2)
- Fractionation of cells (3)
- Isolation of antigen-antibody complex (4)
Contents
Protein A Agarose is supplied as a suspension in buffer containing 0.02 M sodium phosphate, (pH 7.2), 0.15 M sodium chloride, 0.02% (w/v) merthiolate.
Intended Use
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Product Qualification
The Certificate of Analysis provides detailed quality control information for each product. Certificates of Analysis are available on our website at www.invitrogen.com/support.
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References
- Hjelm, H., Hjelm, K., and Sjoquist, J. (1972) FEBS Lett. 28, 73.
- Goding, J.W. (1978) J. Immunol. Meth. 20, 241.
- Ghetie, V., Mota, G., and Sjoquist, J. (1978) J. Immunol. Meth. 21, 133.
- Bauer, K., Bayer, P.M., Deutsch, E., and Gabl, F. (1980) Clin. Chem. 26, 297.
MAN0001295 20-May-2010
