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Introduction
After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. The table below lists PCR primers that can be used in quantitative polymerase chain reactions (qPCR) to measure the expression levels of specific genes for characterizing neural stem cells (NSCs) and their sublineages.
View PCR primers by target
| Target | Primer | Sequence | Tm (°C) | Amplicon size (bp) | Intron size (bp) |
|---|---|---|---|---|---|
| Neural stem cells | SOX1-F | GCGGAAAGCGTTTTCTTG | 53.0 | 406 | No Intron |
| SOX1-R | TAATCTGACTTCTCCTCCC | 50.2 | |||
| SOX2-F | ATGCACCGCTACGACGTGA | 59.3 | 437 | No Intron | |
| SOX2-R | CTTTTGCACCCCTCCCATTT | 56.0 | |||
| NESTIN-F | CAGCGTTGGAACAGAGGTTGG | 58.6 | 389 | 1,142 | |
| NESTIN-R | TGGCACAGGTGTCTCAAGGGTAG | 60.7 | |||
| Oligodendrocytes | MAG-F | TCTGGATTATGATTTCAGCC | 49.7 | 366 | 159 |
| MAG-R | GCTCTGAGAAGGTGTACTGG | 54.7 | |||
| OSP-F | ACTGCTGCTGACTGTTCTTC | 55.1 | 283 | 5,714 | |
| OSP-R | GTAGAAACGGTTTTCACCAA | 50.8 | |||
| Astrocytes | ALDH1L1-F | TCACAGAAGTCTAACCTGCC | 55.5 | 398 | 21,837 |
| ALDH1L1-R | AGTGACGGGTGATAGATGAT | 54.4 | |||
| GFAP-F | GTACCAGGACCTGCTCAAT | 55.0 | 321 | 2,989 | |
| GFAP-R | CAACTATCCTGCTTCTGCTC | 55.3 | |||
| Neurons | MAP2-F | CCACCTGAGATTAAGGATCA | 55.1 | 482 | 11,798 |
| MAP-R | GGCTTACTTTGCTTCTCTGA | 55.0 | |||
| ChAT-F | ACTGGGTGTCTGAGTACTGG | 55.0 | 451 | 7,692 | |
| ChAT-R | TTGGAAGCCATTTTGACTAT | 54.9 | |||
| Endogeneous control | ACTB-F | ACCATGGATGATGATATCGC | 58.2 | 281 | 135 |
| ACTB-R | TCATTGTAGAAGGTGTGGTG | 54.4 | |||
| GABAergic/Glutaminergic neurons | GAD1-F | GTCGAGGACTCTGGACAGTA | 55.3 | 357 | 12,277 |
| GAD1-R | GGAAGCAGATCTCTAGCAAA | 54.9 | |||
| Serotonergic neurons | SLC6A4-F | GCCTTTTACATTGCTTCCTA | 54.8 | 447 | 2,251 |
| SLC6A4-R | CCAATTGGGTTTCAAGTAGA | 55.2 | |||
| Cholinergic neurons | ChAT-F | ACTGGGTGTCTGAGTACTGG | 55.0 | 451 | 7,692 |
| ChAT-R | TTGGAAGCCATTTTGACTAT | 54.9 | |||
| Dopaminergic neurons | TH-F | TCATCACCTGGTCACCAAGTT | 56.0 | 126 | 656 |
| GGTCGCCGTGCCTGTACT | 60.0 |
09-May-2012
