Chick Chorioallantoic Membrane (CAM) Assay
While there have been many variations developed over the years, the basic assay is performed by implanting a membrane or coverslip containing the compound of interest on the chick embryo chorioallantoic membrane through a hole cut in the egg shell. The subsequent incubation period ranges from 1-3 days, depending on the compound, after which time angiogenesis can be quantified via image analysis or colorimetric detection methods.
The CAM assay is quick, technically simple, and inexpensive. However, a major drawback is that it is labor intensive due to the large number of eggs that are required to obtain consistent results. The investigator should note that this is a non-mammalian system which should be taken into consideration when interpreting results.
LT175 updated 7-Oct-2011