Transfection and Selection
Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a variety of research and drug discovery applications. For more information on the most appropriate transfection method for your cell line and application, refer to our Transfection Selection Guide.
Invitrogen offers the most complete collection of cationic lipid-based transfection reagents with exceptional performance that can be used for plasmid delivery into a broad range of cells. Lipofectamine™ LTX with PLUS™ Reagent (Cat. no. 15338-100) is a plasmid DNA-specific transfection reagent that provides maximum expression with minimum cytotoxicity.
Viral Delivery Systems
Having evolved to proficiently deliver nucleic acids to cells, viruses offer a means to reach hard-to-transfect cell types for protein overexpression or knockdown. Adenoviral, oncoretroviral, and lentiviral vectors have been used extensively for delivery in cell culture and in vivo. Invitrogen offers a variety of products used in viral-mediated transduction, including BLOCK-iT™ Adenoviral and Lentiviral Expression Systems.
- For more information and a complete list, refer to our resources on RNAi Transfection
Transient Protein Production & High Throughput Screening
- For more information and a complete list, refer to our resources on Transient Protein Production & High Throughput Screening
Neon™ Transfection System
|The Neon™ Transfection System (Cat. no. MPK5000) is a novel, benchtop electroporation device that employs an electroporation technology by using the pipette tip as an electroporation chamber to efficiently transfect mammalian cells including primary and immortalized hematopoietic cells, stem cells, and primary cells. The Neon™ Transfection System efficiently delivers nucleic acids, proteins, and siRNA into all mammalian cell types including primary and stem cells with a high cell survival rate.|