Cell Culture Protocols
This section provides guidelines and general procedures for routine subculturing, thawing, and freezing of cells in culture.
Methods & Cell Culture Protocols
- Concentrating Cells: A procedure to concentrate cells from suspension culture or to resuspend cells from a monolayer culture.
- Counting Cells in a Hemacytometer: How to count and calculate the number of cells from a stock flask or culture dish.
- Guidelines for Maintaining Cultured Cells: Learn what is subculture and when to do it, review media recommendations for common cell lines and how to dissociate cells as well as the benefits of using TrypLE™ as an alternative to trypsin.
- Subculturing Adherent Cells: A general procedure for subculturing adherent mammalian cells in culture
- Subculturing Suspension Cells: A general procedure for subculturing mammalian and insect cells in suspension culture.
- Freezing Cells: A general procedure to properly cryopreserving cell lines.
- Thawing Frozen Cells: A general procedure for properly thawing cell lines.
- Trypan Blue Exclusion: A rapid assay to accurately determine the viability of your cells.