Amplifying DNA

No matter what the source of DNA, you will most likely need to use PCR to generate sufficient material for cloning. We offer best in-class PCR enzymes with brands you trust including our most popular enzymes for cloning (Table 1).

Hot-start Amplification of a Broad Range of Targets

AmpliTaq Gold® 360 DNA Polymerase (Roche) and Platinum® Taq DNA Polymerase

  • Increased specificity with choice of trusted hot-start technologies
  • Amplify the broadest range of targets (<3 kb)
  • Available in Master Mix or SuperMix formats for increased convenience

Chemically modified hot-start AmpliTaq Gold® 360 DNA Polymerase has been optimized to amplify a wide range of targets while delivering the high specificity, yield, and reliability you have come to expect.

Platinum® Taq DNA Polymerase uses antibody-mediated hot-start to enable increased specificity, yield, and sensitivity with the fastest activation times. Although there are several types of hot-start enzymes, the most reliable and widely used techniques involve chemically modified hot-start or antibody-mediated hot-start enzymes.

How to Choose Between Chemically Modified & Antibody-mediated Hot-start Reagents

Chemically modified hot-start reagents are best when performing high-throughput applications. These enzymes withstand longer periods at room temperature. However, the activation time is longer and the read lengths are shorter. Antibody-mediated hot-start products deliver fast activation times and minimal template degradation, which promote the amplification of longer fragments. However, antibody-mediated chemistries tend to exhibit reduced specificity and involve longer setup times.

With both types of hot-start technologies available from Life Technologies, you can choose the chemistry that works best for your research.

Robust PCR Aplification with High Yields & Increased Fidelity

Platinum® Taq DNA Polymerase High Fidelity

  • Greater than six times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 20 kb
  • High yields with no need to optimize reaction conditions

The accuracy of Platinum® Taq DNA Polymerase High Fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´ > 5´ exonuclease activity) enzyme from Pyrococcus species, GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic “hot-start” technology. Platinum® Taq DNA Polymerase High Fidelity is especially effective for high-fidelity applications such as cloning or mutagenesis.  Platinum® Taq DNA Polymerase High Fidelity predominantly produces PCR products with 3’-A overhangs for compatibility with TOPO® TA Cloning® Kits.

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Which Standard PCR Enzyme Is Right for You?

  Value Choice for non-Hot Start DNA Polymerase Value Choice for non-Hot Start DNA Polymerase General Choice for Hot Start DNA Polymerase Premium Choice for Hot Start DNA Polymerase
Top Seller yes yes
Improved Specificity yes yes
Improved Sensitivity yes
Amplicon Size Up to 5 kb Up to 5 kb Up to 5 kb Up to 8 kb
Improved Yield yes
GC-Rich Format yes yes
Activation Time Immediate Immediate 10 minutes 30 seconds
Master Mix/Super Mix Format yes yes yes yes
Price/Reaction $0.30 - $0.55 $0.44 - $0.55 $0.69
(4,000 Reactions) 		$0.72
(5,000 Reactions)
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