No matter what the source of DNA, you will most likely need to use PCR to generate sufficient material for cloning. We offer best in-class PCR enzymes with brands you trust including our most popular enzymes for cloning (Table 1).
Hot-start Amplification of a Broad Range of Targets
AmpliTaq Gold® 360 DNA Polymerase (Roche) and Platinum® Taq DNA Polymerase
- Increased specificity with choice of trusted hot-start technologies
- Amplify the broadest range of targets (<3 kb)
- Available in Master Mix or SuperMix formats for increased convenience
Chemically modified hot-start AmpliTaq Gold® 360 DNA Polymerase has been optimized to amplify a wide range of targets while delivering the high specificity, yield, and reliability you have come to expect.
Platinum® Taq DNA Polymerase uses antibody-mediated hot-start to enable increased specificity, yield, and sensitivity with the fastest activation times. Although there are several types of hot-start enzymes, the most reliable and widely used techniques involve chemically modified hot-start or antibody-mediated hot-start enzymes.
How to Choose Between Chemically Modified & Antibody-mediated Hot-start Reagents
|Chemically modified hot-start reagents are best when performing high-throughput applications. These enzymes withstand longer periods at room temperature. However, the activation time is longer and the read lengths are shorter.||Antibody-mediated hot-start products deliver fast activation times and minimal template degradation, which promote the amplification of longer fragments. However, antibody-mediated chemistries tend to exhibit reduced specificity and involve longer setup times.|
With both types of hot-start technologies available from Life Technologies, you can choose the chemistry that works best for your research.
Robust PCR Aplification with High Yields & Increased Fidelity
Platinum® Taq DNA Polymerase High Fidelity
- Greater than six times higher fidelity than Taq DNA polymerase
- Amplification of fragments up to 20 kb
- High yields with no need to optimize reaction conditions
The accuracy of Platinum® Taq DNA Polymerase High Fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´ > 5´ exonuclease activity) enzyme from Pyrococcus species, GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic “hot-start” technology. Platinum® Taq DNA Polymerase High Fidelity is especially effective for high-fidelity applications such as cloning or mutagenesis. Platinum® Taq DNA Polymerase High Fidelity predominantly produces PCR products with 3’-A overhangs for compatibility with TOPO® TA Cloning® Kits.
Which Standard PCR Enzyme Is Right for You?
|Value Choice for non-Hot Start DNA Polymerase||Value Choice for non-Hot Start DNA Polymerase||General Choice for Hot Start DNA Polymerase||Premium Choice for Hot Start DNA Polymerase|
|Amplicon Size||Up to 5 kb||Up to 5 kb||Up to 5 kb||Up to 8 kb|
|Activation Time||Immediate||Immediate||10 minutes||30 seconds|
|Master Mix/Super Mix Format|
|Price/Reaction||$0.30 - $0.55||$0.44 - $0.55||$0.69
|Order Now||Order Now||Order Now||Order Now|