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In This Issue
GET CONNECTED
| DEPARTMENTS
UPCOMING MEETING
OTHER PUBLICATIONS
BIOPROBES® JOURNAL IS GOING GREEN
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FEATURED NEW PRODUCTS
Identify Mouse Antibody Subtypes Reliably—New Mouse Immunoglobulin Isotyping Kit
| what it is
| how it works
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| Product | Quantity | Cat. No. | |
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| Mouse Immunoglobulin Isotyping Kit | 6 plates | 992000 |  |
Fluorogenic pH Sensors for Live-Cell Analyses—pHrodo™ Red Labeling Kit and pHrodo™ Red Avidin
| what they are
| how they work
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| Product | Quantity | Cat. No. | |
|---|---|---|---|
| pHrodo™ Red Microscale Labeling Kit, 3 labeling reactions | 1 kit | P35363 | |
| pHrodo™ Red Avidin | 1 mg | P35362 | |
New Recombinant Antibodies—LRRK2 ABfinity™ Antibodies
| what they are
| how they work
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| Product | Quantity | Cat. No. | |
|---|---|---|---|
| LRRK2 [pS935] ABfinity™ Recombinant Rabbit Oligoclonal Antibody | 100 µg | 710097 | |
| LRRK2 [pS935] ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified | 100 µg | 701066 | |
Investigate p70S6 Kinase Up-regulation—New Phospho–p70S6 Kinase [pT421/pS424] Recombinant Oligoclonal Antibody
| what they are
| how it works
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| Product | Quantity | Cat. No. | |
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| p70S6 Kinase [pT421/pS424] Recombinant Rabbit Oligoclonal Antibody | 100 µg | 710108 | |
NEW APPLICATIONS
CellLight® Reagents in Translational Medicine
CellLight® reagents are ready-to-use BacMam viral particles containing fluorescent protein constructs targeted to specific subcellular structures. They are simple and effective tools for introducing targeted intracellular fluorescent labels into living cells. Simply add the CellLight® reagent to your cells, incubate overnight, and you’re ready to image your cells.
- Efficient transduction of mammalian cells, including “difficult” cells such as primary and stem cells
- Robust expression and sustained cellular function of fluorescent proteins for highly specific labeling
- Easily study dynamic changes, colocalization, and signaling events in living cells
- Compatible with fixed-cell staining and high-content analysis
In a recent study, Gasparian et al. published results that help elucidate the mode of action of a class of small molecules, curaxins, that have the potential to be useful in cancer therapy (Sci Transl Med 3, 95ra74 (2011)). They found that this newly identified class of anti-cancer compounds exhibits activity in mice at levels that are not toxic or genotoxic—an unusual property for DNA-targeted chemotherapeutics. In addition, they show that these compounds “simultaneously activate p53 and inhibit NF-κB”, proteins that are dysregulated in many malignant tumor types. CellLight® Histone 2B-RFP, BacMam 2.0 was used in this study to help show that curaxins cause functional inactivation of the FACT (facilitates chromatin transcription) complex by causing one of its two subunits, structure-specific recognition protein 1 (SSRP1), to become tightly associated with chromatin.
- Learn More About CellLight® Ready-to-Use Fluorescent Protein–Based Reagents
PROVEN PERFORMERS
Easy, Reliable Cell Counting of 120 Different Cell Types and Counting—Countess® Automated Cell Counter
Accurate—without the subjectivity of manual cell counting and user-to-user variability- Fast—total cell counts, percent viable cells, and average cell size in as little as 30 seconds
- Versatile—can be used with a wide variety of cells: documented with 120 different cell types from 18 species so far
- Convenient—requires no cleaning or routine maintenance, and minimal setup
Have confidence in your cell counts with the Countess® Automated Cell Counter, which has been referenced in over 100 peer-reviewed publications representing over 120 different cell types from 18 species. The Countess® Automated Cell Counter is a benchtop instrument that offers easy and accurate cell and viability counts in as little as 30 seconds. This image-based cell counting instrument eliminates the tedium and subjectivity of manual cell counting and minimizes subjective judgments that can lead to errors. By offering accurate cell count, cell size, and cell viability data, the Countess® instrument aids in improving downstream results, which translates to fewer experimental repeats needed due to subjective errors. To supplement the peer-reviewed publications, we have generated several technical and application notes covering topics such as counting blood, primary cells, and stem cells.
The Countess® Automated Cell Counter continues to set the standard for benchtop cell counters.
- Learn More About the Countess® Automated Cell Counter
DEPARTMENTS
On the Web
 | Introducing the FLoid™ Cell Imaging Station — from vessel to image in as few as 2 clicks Find out how simple it is to collect fluorescent cell images using the new FLoid™ Cell Imaging Station. |
Imaging Corner
 | click to enlarge | |
| Live HeLa cell imaging. Live HeLa cells were transduced with CellLight® Plasma Membrane-GFP, BacMam 2.0 construct (green) and wheat germ agglutinin, Alexa Fluor® 594 conjugate (red), both targeting the plasma membrane. The cells were then counterstained with Hoechst 33342 dye (blue) to label nuclei, and imaged live. |
From the Bench
Lee MS, Cherla RP et al. (2011) Cell Microbiol 13:11479-96
There have have been several outbreaks of deadly Escherichia coli in the last few years; the US Centers for Disease Control and Prevention (CDC) lists six such events in 2010 and 2011, with the sources of contamination covering a wide range of food products that include beef, lettuce, cheese, and nuts. Shiga toxins produced by certain strains of E. coli and Shigella dysenteriae are responsible for the severe illness and death that can result when humans become infected with these bacteria. It is hoped that research aimed at better understanding the effect of shiga toxins on host cells will help identify strategies for effective treatment and/or prevention of illness. To that end, scientists at the Texas A&M Health Science Center and colleagues recently compared intracellular trafficking and autophagosome formation of shiga toxin in toxin-sensitive and toxin-resistant cells. They were particularly interested in understanding whether autophagy in response to shiga toxin is a protective mechanism or whether it contributes to apoptosis. They found that shiga toxin was translocated to different cell compartments in toxin-sensitive and -resistant cells. In addition, they report that both cell types exhibited autophagosome formation. They determined that “proteolytic cleavage of Atg5 and Beclin-1 plays pivotal roles in switching non-cytotoxic autophagy to cell death signalling.”
- Gibco® cell culture media and sera were used in this study
- Premo™ Autophagy Sensors were used for detection of autophagy
- Alexa Fluor® 488 and 594 dye–labeled shiga toxin fragments were used to investigate intracellular trafficking
