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FEATURED NEW PRODUCTS
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FEATURED NEW PRODUCTS
what they are
CellLight™ reagents are fluorescent protein–signal peptide fusions that permit accurate and specific targeting to cellular structures, including the cytoskeleton, for live-cell imaging applications, or for fixed-cell analyses following formaldehyde-based fixation.
what they offer
how they work
Cellular labeling with CellLight™ reagents employs BacMam technology, which uses a modified insect cell baculovirus coupled with a mammalian promoter as a vehicle to efficiently deliver and express genes in mammalian cells. The inability of baculoviruses to replicate in mammalian cells renders them safe as research reagents and provides a transient, footprint-free method to label cells. Unlike expression vectors, BacMam reagents enable titratable and reproducible expression and offer high cotransduction efficiency, enabling multiple BacMam reagents to be used in the same cell.
|CellLight™ Actin-GFP *BacMam 2.0*||1 mL||C10582|
|CellLight™ MAP4-GFP *BacMam 2.0* ||1 mL ||C10598|
|CellLight™ MAP4-RFP *BacMam 2.0*||1 mL||C10599|
|CellLight™ Talin-GFP *BacMam 2.0* ||1 mL ||C10611|
|CellLight™ Talin-GFP *BacMam 2.0*||1 mL ||C10612|
|CellLight™ Tubulin-GFP *BacMam 2.0* ||1 mL ||C10613|
|CellLight™ Tubulin-RFP *BacMam 2.0*||1 mL||C10614|
what they are
CellLight™ reagents provide a simple and effective method for introducing targeted intracellular labels into live cells, enabling unambiguous visualization by fluorescence microscopy of key organelles involved in membrane trafficking.
what they offer
how they work
BacMam technology is based on the use of an insect cell baculovirus for efficient gene delivery and expression in mammalian cells. The enhanced BacMam 2.0 system greatly expands the efficiency and utility of this gene delivery platform. Cell types previously not compatible with or poorly transduced with BacMam 1.0 can now be labeled with BacMam 2.0, including primary cells (e.g., neurons) and stem cells. This improved performance is due to inclusion of elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, and genetic elements that boost expression levels.
|CellLight™ ER-GFP *BacMam 2.0*||1 mL||C10590 |
|CellLight™ ER-GFP *BacMam 2.0*||1 mL||C10591|
|CellLight™ Golgi-GFP *BacMam 2.0*||1 mL||C10592|
|CellLight™ Golgi-RFP *BacMam 2.0*||1 mL||C10593|
what it is
Explore the fascinating world of cellular imaging with this new online tool. Select different combinations of cellular structures and fluorophores to create your version of the perfectly labeled fluorescent cell!
what it offers
how it works
Stain your own cell virtually, using our brand-new cell staining simulation tool to develop reproducible results with many of our signature fluorescent dyes.
The avidin–pHrodo™ dye conjugate has been used to visualize tumors with high target-to-background ratios after injection into tumor-bearing mice. A unique advantage of this approach over other molecular imaging methods is that the fluorescent probe is quenched when injected, and is activated only after binding and internalization by the target cancer cells or tissues.
Learn More about pHrodo™ Indicators
Fluorescence imaging of peritoneal tumors in mice. The avidin–pHrodo™ dye conjugate was injected into SHIN3 peritoneal tumor-bearing mice (50 μg, i.p.). One hour after the probe injection, fluorescence endoscopy (A) was performed using the Olympus EVIS ExERA-II CLV-180 system (Olympus Corp., Tokyo, Japan; excitation wavelength, 543/25 nm, emission filter, 597.5/55 nm). After endoscopy the mice were sacrificed, and ex vivo fluorescence spectral imaging (B) was performed on a Maestro In-Vivo Imaging System (CRi, Inc., Woburn, MA; excitation filter, 503 to 555 nm; emission filter, longpass filter over 580 nm). The spectral fluorescence images consisting of autofluorescence spectra and the spectra from the pHrodo™ dye were obtained and then unmixed based on their spectral patterns. Tumors were clearly visualized with low background signal, and the results were consistent with the endoscopy. Images reproduced with permission from Mikako Ogawa and coworkers, National Cancer Institute.
Liu Y, Sato F, Kawamoto T et al. (2010) Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells. Genes Cells 15:315–325.
In a study to define the roles of the transcription factors DEC2 and DEC1 in the survival of tumor cells, Liu and colleagues carried out several experiments to alter the levels of DEC2 and DEC1 in the MCF-7 human breast cancer cell line. Following siRNA knockdown of DEC2, cell death was markedly enhanced and nuclear condensation was observed (using the Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay and Hoechst 33258 dye). These observations are consistent with the role of DEC2 as an apoptosis suppressor. In contrast, knockdown of DEC1 did not affect cell survival. Overexpression of DEC2 reduced the amount of cleaved PARP and caspase-8 (induced by TNF-alpha treatment), whereas overexpression of DEC1 increased the levels of these same proteins (with or without TNF-alpha treatment)—again supporting the hypothesis that DEC2 and DEC1 have opposing properties with respect to apoptosis. The authors conclude that the balance between the levels of DEC2 and DEC1 directly translates to the balance of survival of MCF-7 cells.
|Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay||1 kit||C10245|
|Hoechst 33258, pentahydrate (bis-benzimide), FluoroPure™ grade||100 mg||H21491|
Visualizing the cytoskeleton and Golgi complex in African green monkey kidney fibroblast cells. COS-7 cells were labeled with Alexa Fluor® 488 phalloidin (green), Alexa Fluor® 350–wheat germ agglutinin conjugate (blue), and propidium iodide (red). Image contributed by Mike Davidson, Florida State University.
|Product||Quantity||Cat. No. |
|Alexa Fluor® 488 Phalloidin||300 units|
|Propidium Iodide||100 mg|
|Wheat Germ Agglutinin, Alexa Fluor® 350 conjugate ||5 mg |
Tracking Tumor Cell Migration With CellTracker™ Dyes
Molecular Probes® CellTracker™ dyes freely diffuse through the membranes of live cells and, once inside, the action of intracellular esterase converts them to fluorescent forms that survive fixation. Cells labeled in this manner remain viable and fluorescent for days to weeks, even after 5–6 divisions. For cell migration studies, these dyes can be used to follow cells as they migrate through tissue, either artificial or real, and can be multiplexed with functional probes for the simultaneous detection of cell migration and ECM degradation. CellTracker™ dyes have been used to visualize the migration of tumor cells through three-dimensional extracellular matrices, their interaction with stromal fibroblasts, and the simultaneous digestion of the ECM protein collagen IV.
- Learn More about Cell Tracing and Tracking
Interaction of tumor spheroids with stromal fibroblasts results in extensive degradation of type IV collagen. HCT116 human colon carcinoma cells were cocultured with human colon fibroblasts prestained with CellTracker™ Orange in reconstituted basement membrane containing DQ™ collagen IV. Extensive proteolysis resulting in fluorescence of DQ™ collagen IV (green) occurs at the site of interaction between the tumor spheroids and the fibroblasts (orange), with yellow regions denoting areas of overlap. Magnification: 40x. Image supplied by Drs. Mansoureh Sameni and Bonnie Sloane, Wayne State University.
Imaging tumor cell migration in vitro. Tumor cells are labeled with CellTracker™ dye and migrate through DQ™ collagen substrate suspended in three-dimensional matrix gel. Green fluorescence indicates the region of collagen degradation by secreted proteases. In this schematic, cells are labeled orange with CellTracker™ Orange dye, and nuclei are stained blue with Hoechst 33342.
|CellTracker™ Blue CMAC ||5 mg ||C2110|
|CellTracker™ Green CMFDA||20 x 50 mg||C7025|
|CellTracker™ Orange CMTMR||1 mg||C2927|
|CellTracker™ Orange CMRA||20 x 50 mg||C34551|
|CellTracker™ Red CMTPX||20 x 50 mg||C34552|
|DQ™ Collagen, type IV from human placenta, fluorescein conjugate||1 mg||D12052|
|Hoechst 33342, 10 mg/mL solution in water||10 mL||H3570|
Labeling Chemistry Selection Tool—NEW!
Quickly find the best reactive dye or label for your experiment. Whether you are labeling proteins and antibodies for immunofluorescence, nucleic acids for in situ hybridization, or lipids for membrane studies, this tool will help you find the right conjugation product.
Learn More about the Labeling Chemistry Selection Tool
Molecular Probes® The Handbook
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