Sensitive and Specific Detection
Invitrogen™ anti–fluorescent protein antibodies are also consistent and reliable tools for detecting fluorescent proteins and fluorescent protein fusions in western blot and flow cytometry applications. These antibodies are highly specific—they only react with the intended target—which contributes to low background signal and increases signal to noise.
Validated for a Range of Applications
Figure 1. Immunolabeling enhances GFP fluorescence. HeLa cells were transduced with CellLight® Lysosomes-GFP, then fixed and permeabilized. GFP, localized in the lysosomes, was labeled with anti-GFP rabbit monoclonal antibody and detected with Alexa Fluor® 594 goat anti–rabbit IgG (H+L) (red). The nucleus was stained with DAPI (blue), and the sample was mounted using ProLong® Gold antifade reagent. (Left panel) Some GFP fluorescence was retained in the lysosomes after fixation. (Center panel) Immunolabeling and detection greatly improved visualization by enhancing the dim GFP fluorescence. (Right panel) The merged yellow signal indicates colocalization of GFP fluorescence and the detection antibody.
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