Highly Selective Antibodies for GFP and RFP

Highly Selective Antibodies for GFP and RFP

Fluorescent proteins are highly versatile biological markers for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. To complement these powerful tools, we offer anti-GFP and anti-RFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. All six of our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and most GFP fusion proteins. The affinity-purified anti-RFP antibody is used to detect native TagRFP and most fusion proteins derived from Entacmaea quadricolor.

Sensitive and Specific Detection

Anti-GFP and anti-RFP antibodies provide a convenient method for visualizing GFP and RFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. Our anti-GFP and anti-RFP antibodies are easily incorporated into standard immunostaining protocols for cell and tissue analysis (Figure 1).

Invitrogen™ anti–fluorescent protein antibodies are also consistent and reliable tools for detecting fluorescent proteins and fluorescent protein fusions in western blot and flow cytometry applications. These antibodies are highly specific—they only react with the intended target—which contributes to low background signal and increases signal to noise.

Validated for a Range of Applications

Invitrogen™ antibodies have been validated for a broad range of applications and targets. Find antibodies for your application.

Figure 1. Immunolabeling enhances GFP fluorescence. HeLa cells were transduced with CellLight® Lysosomes-GFP, then fixed and permeabilized. GFP, localized in the lysosomes, was labeled with anti-GFP rabbit monoclonal antibody and detected with Alexa Fluor® 594 goat anti–rabbit IgG (H+L) (red). The nucleus was stained with DAPI (blue), and the sample was mounted using ProLong® Gold antifade reagent. (Left panel) Some GFP fluorescence was retained in the lysosomes after fixation. (Center panel) Immunolabeling and detection greatly improved visualization by enhancing the dim GFP fluorescence. (Right panel) The merged yellow signal indicates colocalization of GFP fluorescence and the detection antibody.