One Reaction, Myriad Possibilities
Nascent RNA obtained using the Click-iT® Nascent RNA Capture Kit has been validated for use in both TaqMan® Assay– and SYBR® Green dye–based qPCR, microarray analysis, and sequencing using the SOLiD™ System. Applications include analysis of gene expression, RNA stability, regulation of transcription; and rates of RNA synthesis, decay, and degradation.
Boost the Resolution of Your Gene Expression Analysis
Figure 1. High-resolution gene expression analysis with the Click-iT® Nascent RNA Capture Kit. Relative expression (ΔCt) of the indicated genes was compared with and without a treatment thought to affect apoptosis (ΔΔCt). For many transcripts, the change in relative gene expression measured was dramatically enhanced when nascent RNA was used for the analysis rather than total RNA.
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Discovery, Not Just Recovery
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