• High biological activity
• High purity
• Proven compatibility with Gibco® media

Gibco® recombinant proteins are highly purified bioactive proteins. Gibco® cytokines and receptors have been bioassayed with Gibco® media. Get your recombinant proteins from the media company you use and trust.

TNF-α, TNF-β/lymphotoxin, and TRAIL are proinflammatory cytokines that activate various signaling pathways, including the NF-κB signaling pathway. Upon TNF-α stimulation, IκBα is phosphorylated, which marks the protein for degradation, making NF-κB free to translocate to the nucleus to induce gene expression.

We offer bioactive recombinant TNF-α from human, mouse, rat, and swine for use in cell culture applications. We also offer recombinant human TNF-β/lymphotoxin, TRAIL, and soluble forms of TNF-RI and TNF-RII.

Gibco® cytokines and growth factors are high-purity recombinant proteins with high bioactivity. To help ensure Gibco® recombinant proteins are of the highest quality, each protein is analyzed for purity and structural homogeneity.

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• ABfinity™ TRAF3 rabbit recombinant monoclonal for sensitive detection
• Consistent performance for your assays
• Consistent product from lot to lot

ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including TRAF3.

TNF receptor-associated factor 3 (TRAF3) is important in regulating immune responses in numerous pathways downstream of TNF signaling cascades, such as JNK and NFkB in T cell–mediated immunity.

ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway reagents. ABfinity™ technology helps ensure consistent antibody performance, so you don’t have to reoptimize your assay with each new lot.

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• Superior performance—enables accurate quantitation of TNF signaling pathway proteins
• High quality—fully characterized and validated antibodies that deliver high specificity and sensitivity
• Fast and easy—NEW magnetic bead–based Luminex® assays

We have developed analysis platforms and assay kits to measure a wide range of cytokine and signal transduction proteins. The Invitrogen™ Luminex® 200™ and FlexMAP 3D® systems are compact benchtop analyzers capable of performing up to 100 assays simultaneously in a single well of a 96-well microtiter plate (Luminex® 200™ system)

or up to 500 assays simultaneously in a single well of either a 96-well or 384-well microtiter plate (FlexMAP 3D® system).

Invitrogen™ Luminex® singleplex and multiplex assays provide a rapid and cost-effective alternative approach to the analysis of numerous key pathway proteins. These assays can be applied to a broad range of species (human, mouse, rat, and monkey) and sample types (serum, plasma, and tissue culture media). All lots of Invitrogen™ Luminex® singleplex and multiplex assays have been qualified using the Invitrogen™ Luminex® 200™ System and the FlexMAP 3D® System, which helps ensure superior analysis performance and reproducible results.

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• Reliable measurements of TNFα in serum, plasma, or supernatant
• Fast assay—as little as 2.5 hours of incubation time
• Consistent results

Tumor Necrosis Factor Alpha (TNF-α), also called cachectin, is a cytokine mainly produced by monocytes and activated macrophages. TNF-α is defined by its participation in inflammatory activities, cell toxicity, and mediation of TNF-α tumor toxicity.

Accurate and precise measurements of TNF-α are critical in many research areas. Invitrogen™ ELISA kits provide an easy and reliable way to quantify the amount of TNF-α in your serum, plasma, or supernatant sample. Precoated ELISA kits are available with various species reactivities, including human, mouse, rat, swine, and monkey. Economy pack sizes are also available for your convenience. For more savings and flexibility to develop your own immunoassays, matched antibody pairs are also available.

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