FAK is under investigation in several areas of research, including studies of cell adhesion and migration, growth factor–mediated migration, differentiation, cell cycle, apoptosis, and cancer. FAK is overexpressed in breast, colon, and thyroid cancers, possibly contributing to some features of the malignant phenotype (i.e., increased migration and invasion of surrounding stroma).
Integrins play an important role in cell attachment to other cells or to the extracellular matrix as well as in signal transduction. Focal adhesion kinase (FAK) is a nonreceptor protein tyrosine kinase that localizes to focal adhesions. In response to integrin engagement, FAK is autophosphorylated at tyrosine residue 397. This autophosphorylation event creates high-affinity binding sites for the SH2 domains of several important signaling proteins.
Our phosphoELISA™ kits offer the ability to quickly measure signaling proteins in cell lysates using a convenient 96-well format. Just process your samples as you would for a western blot, then add them to an ELISA plate, run the assay, and get quantitative results in as little as 4 hours.
ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including p53 (gene ID 23491729).
p53 can drive tumor progression through integrin recycling. p53 can disrupt direction of migration and other metastatic characteristics through integrin and epidermal growth factor (EGFR) signaling. ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway research. ABfinity™ technology helps to ensure consistent antibody performance, so you don’t have to reoptimize your assay with each new lot.
|Product ||Reactivity||Quantity ||Cat. No. |
|p53 [pS15] ABfinity™ Recombinant Rabbit Monoclonal Antibody, purified ||Human||100 µg||700439|
|Mouse anti-p53, unconjugated monoclonal||Human||200 µg||134000|
|Mouse anti-p53, unconjugated monoclonal||Human||100 µg||134100|
|p53 [pS392] Rabbit Polyclonal Antibody, unconjugated||Rat, mouse, human||10 blots||44640G|
|Mouse anti-p53, unconjugated monoclonal||Mouse||100 µg||AHO0032|
|Mouse anti-p53, unconjugated monoclonal||Bovine, human||100 µg||AHO0152|
|Alexa Fluor® 488 Goat Anti-Rabbit||Rabbit||0.5 mL||A11008|
Gibco® recombinant proteins are useful for a wide variety of pathway research investigations. Gibco® recombinant proteins have been bioassayed in cells cultured in Gibco® media. So if you’re already using Gibco® media, you can have more confidence in the compatibility of these reagents in your cell culture–based assays.
Integrins are important in transmitting signals from the extracellular matrix (ECM) to cells. The ECM is involved in movement of cells during wound healing and embryonic development, and also relays important information to the cells. Two key ligands found in the ECM are fibronectin and vitronectin. These glycoproteins bind to integrins at the surface of the cell and play a role in a variety of biological processes.
Fibronectin plays a role in cell adhesion, growth, migration, and differentiation and is involved in diverse processes impacting wound healing, development, cancer, and fibrosis.
Vitronectin plays a role in cell adhesion and binds to serpin serine protease inhibitors. It may be involved in cancer and hemostasis.
Gibco® cytokines, growth factors, and proteins are high-purity recombinant proteins with high bioactivity. To help ensure Gibco® proteins are of the highest quality, each protein is analyzed for purity along with structural homogeneity so that a biologically active protein is produced.
Our competitive, heterogeneous cAMP-Screen® and cAMP-Screen Direct® immunoassays measure cAMP directly from cell lysates at levels as low as 60 fmol to 6 µmol, over 4 to 5 logs, without requiring any sample dilution.
Detection is complete typically within 90 minutes, utilizes chemiluminescence, and is amenable to use in automated, high-throughput screening applications.
Cyclic adenosine monophosphate (cAMP) is an important second messenger in many signal transduction pathways linking activation of cell surface membrane receptors to intracellular responses and, ultimately, to changes in gene expression.
Several proteins, including Epac proteins (exchange protein directly activated by cAMP) and Rap1, involved with integrin-mediated T cell adhesion, are directly modulated by cAMP.
The sensitivity and simplicity of the cAMP-Screen Direct® assay enables researchers to measure small changes in cAMP levels as they investigate integrin-mediated T cell adhesion.
Through superior performance, Alexa Fluor® dyes have been contributing to illuminating research for over 10 years. And to help with experiment planning, Alexa Fluor® dyes are supported by an experienced technical support team, >30,000 published references, application and experimental tips, and protocols.
Integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals for cell shape, mobility, and
progression through the cell cycle. After incubating the sample with the anti-integrin primary antibody of your choice, you can detect it with any of a number of fluorescent Alexa Fluor® dye–labeled secondary antibodies.
Because of their superior brightness and photostability, Alexa Fluor® conjugates are superior to most conventional fluorescent secondary reagents and are the detection reagents of choice for many fluorescence-based immunoassays.
|Product ||Qty.||Cat. No. |
|Alexa Fluor® 594 goat anti–mouse IgG (H+L), 2 mg/mL ||0.5 mL||A11005|
|Alexa Fluor® 488 Monoclonal Antibody Labeling Kit, 5 labelings||1 kit||A20181|