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Nuclear factor-kappa B (NF-κB) transcription factors are protein dimers composed of various combinations of members of the NF-κB/Rel protein family. Through its regulation of NF-κB, inhibitor of nuclear factor-κB α isoform (IκBα) controls immune and inflammatory responses, cell division, and apoptosis.
NF-κB mediates inflammatory responses, immune responses, responses to viral infections, cell division, and regulation of apoptosis, serving both as an anti-apoptotic signal and a pro-apoptotic signal.
Disease states in which NF-κB is improperly regulated include cancer, neurodegenerative diseases, and numerous diseases related to immune system dysregulation such as arthritis, asthma, and inflammatory bowel disease.
ELISA technology allows a more detailed understanding of proteins in specific NF-кB pathways. We offer sandwich ELISA kits to quickly detect and quantify transcription factors in cell lysate models. These ELISA kits allow high throughput of samples in 96-well format and provide reproducible, quantitative results. Calibrated standard curves are provided to quantify the level of protein in each experimental run.
We offer highly specific ABfinity™ antibodies for NF-κB signal transduction research. These antibodies are validated against a variety of species for most applications.
We also have more than 300 phosphorylation site–specific antibodies available that have been validated with peptide competition experiments. This offering includes antibodies that recognize NF-κB, RelB, TRAF6, IκBα, or related pathways such as Akt, TLC, and FAK.
|Target Protein||Clonality, Clone (Isotype) ||Reactive Species||Applications||Quantity||Cat. No.|
|NFκB (p50) ||pAb, ZK50 (Rb IgG) ||Hu||E, EMSA, WB||100 µg||513500|
|NFκB (p65) ||mAb, 2A12A7 (Ms IgG2a) ||Hu||E, EMSA, WB||100 µg||339900|
|NFκB (p65) ||mAb, 572 (Ms IgG1) ||Hu, Ms (B, Cn, Cp, Eq, Mk (Rh), Rt)||IF, IP, WB||100 µg||436700 |
|NFκB [pS529] ||pAb (Rb IgG) ||Hu||WB||10 blots||44711G|
|RelB ||mAB 17.3 (Ms IgG1) ||Hu||IF, IP, WB||100 µg||437500 |
|IκB [pS32] ||mAb (Rb IgG) ||Hu (Ms, Sw)||E, WB||100 µL||44725G|
E = ELISA; EMSA = electrophoretic mobility shift assay; IF = immunofluorescence; IP = immunoprecipitation; WB = western blot.
B = bovine; Cn = canine; Cp = chimpanzee; Eq = equine; Hu = human; Mk (Rh) = monkey (Rhesus); Ms = mouse; Rb = rabbit; Rt = rat; Sw = swine.