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Pathway Focus: The Akt Pathway
ABfinity™ Recombinant Rabbit Monoclonal Antibodies represent the next generation of antibody detection tools. Produced by expressing specific cloned antibody genes in a mammalian expression system, ABfinity™ antibodies offer improved specificity and more reproducible results.
The PI3K/Akt pathway is associated with major cell functions such as cell growth, survival, apoptosis, and angiogenesis.
ABfinity™ antibodies targeting the PI3K/Akt pathway provide a critical tool for researchers investigating various diseases, including cancer, diabetes, cardiovascular conditions, and neurological disorders.
ABfinity™ antibodies have been tested for reactivity in multiple species and in multiple applications, so researchers can have greater confidence in the results. And each lot of our phosphospecific antibodies is tested in peptide competition experiments to help ensure that the antibody recognizes only the phosphorylated form of the protein.
|Description ||Reactivity: Tested (Predicted) ||Applications ||Quantity||Cat. No. |
|PKC-θ [pT538] ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu (X, Rt, Ms, Cp, B) ||WB, F, IHC, IF/ICC||100 µg||700043|
|Mnk1 [pT197/pT202] ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu (Z, X, Sw, Rt, P, Ms, Mk (Rh), Eq, Eq, Cp, Ch, Cn, B) ||WB, F, IF/ICC||100 µg||700242|
|Cul-2 ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu, Rt, Ms (X, Or, Mk (Rh), Eq, Cp, Cn, B) ||WB, F, IHC, IF/ICC||100 µg||700179 |
|IRAK4 ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu (Sw, Sh, Rt, Qu, Eq, Cn, B) ||F, IF/ICC||100 µg||700026|
|AMPKβ1 [pS182] ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu (X, Rt, Or, Eq, Ch, Cn, B, Ms) ||WB, F, IHC, IF/ICC||100 µg||700241 |
|Smad1/5 [pS463/pS465] ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu (Z, X, Sw, Sh, Su, Rt, Ms, Mk (Rh), Eq, Cp, Ch, Cn, B) ||F, IHC, IF/ICC||100 µg||700047|
|4E-BP1 [pT37] ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu (Z, Rt, Ms, B, Hu, Eq) ||E, WB, F, IHC, IF/ICC||100 µg||700238|
|STAT4 ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu, Ms, Rt (Sw, Eq) ||E, WB, F, IHC, IF/ICC||100 µg||700185 |
|SUMO-3 ABfinity™ Recombinant Rabbit Monoclonal Antibody ||Hu, Ms, Rt (Mk (Rh), Cp) ||WB, F, IHC, IF/ICC||100 µg||700186|
|CASP3 [D175] ABfinity™ Recombinant Rabbit Monoclonal Antibody (clone 9H19L2) ||Hu (X, Sw, Sh, Rt, Rabbit, P, Ms, Eq, Ha, Fe, Eq, Cp, Cn, B) ||E, WB, F, IHC, IF/ICC||100 µg||700182 |
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Currently, Akt is one of the most actively studied kinases or kinase pathways in both the basic research and drug development arenas. Activated Akt plays a key role in mediating signals for cell growth, cell survival (anti-apoptotic), cell-cycle progression, differentiation, transcription, translation, and glucose metabolism.
Get accurate quantitation of 7 Akt pathway proteins with the AKT Total and Phospho 7-Plex Panel. The AKT 7-Plex Panel reports levels of protein, requires a small sample size, and can be run in ~4 hours.
Validation includes bead and protein specificity, precision (intra- and inter-assay, 10%), parallelism between recombinant and natural samples, linearity of dilution, recovery (>80%), and comparison to ELISA (>90%). Premixed Multiplex Kits include General Buffer Reagent Kit components and Antibody Bead Kit components, with reagents premixed for the number of markers in the assay.
Learn More about our Multiplex Protein Assays for Akt Pathway Research
|Akt Pathway Phospho 7-Plex Panel ||Hu, Ms, Rt||Akt [pS473], GSK-3β [pS9], IRS-1 [pS312], IGF-1R [pY1135/pY1136], IR [pY1162/pY1163], p70S6K [pTpS421/424], PRAS40 [pT246]||LHO0001|
|Akt Pathway Total 7-Plex Panel ||Hu, Ms, Rt||Akt, GSK-3β, IGF-1R, IR, IRS-1, p70S6K, PRAS40||LHO0002|
AKT, also known as protein kinase B a (PKB a) or RAC PKa, was initially identified as one of the downstream targets of PI3 kinase (PI3K). Eukaryotic initiation factor 4E binding protein 1 (4E-BP1) is an important effector of mTOR signaling. Hypophosphorylated 4E-BP1 acts as a translational repressor by binding and inhibiting the eIF4E. Proline-rich AKT substrate of 40 kDa (PRAS40) is a direct substrate for AKT.
AKT can be activated by a diverse array of growth factors and physiological stimuli in a PI3K- dependent manner.
Activated AKT affects downstream markers such as 4E-BP1 and PRAS40, and acts as a key mediator of signals for cell survival, proliferation, and angiogenesis.
Invitrogen offers sandwich ELISA kits to quickly detect and quantify phosphorylation site–specific proteins in cell lysates. Total protein kits are provided to normalize the protein content of the sample, independent of the phosphorylation state. Our ELISA kits deliver quantitative results and give you workflow efficiencies with high sample throughput and a 96-well plate format. Results are achieved after as little time as 4 hours of incubation, and assays require less sample than western blots because of the improved sensitivity.
|Product ||Species ||Quantity ||Cat. No. |
|Akt [pS473] ELISA Kit ||Hu, Ms, Rt ||96 tests||KHO0111|
|Akt [pS473] High Sensitivity ELISA Kit ||Hu, Ms, Rt ||96 tests||KHO0541|
|Akt [pT308] ELISA Kit ||Hu ||96 tests||KHO0201 |
|Akt (Total) ELISA Kit ||Hu, Ms, Rt ||96 tests||KHO0101|
|4E-BP1 [pT46] ELISA Kit ||Hu, Ms, Rt ||96 tests||KHO0691 |
|4E-BP1 (Total) ELISA Kit ||Hu, Ms, Rt ||96 tests||KHO0681|
|PRAS40 [pT246] ELISA Kit ||Hu ||96 tests||KHO0421|
|PRAS40 (Total) ELISA Kit ||Hu ||96 tests||KHO0411 |
This enables multiple BacMam reagents to be used in the same cell (including primary and stem cells) for live-cell imaging and for fixed-cell analyses following formaldehyde-based fixation.
|Product ||Cat. No. |
|Premo™ Autophagy Sensor LC3B-GFP *BacMam 2.0* ||P36235|
|Premo™ Autophagy Sensor LC3B-RFP *BacMam 2.0* ||P36236|
|LC3B Antibody Kit for Autophagy ||L10382|
|CellLight® Golgi-GFP *BacMam 2.0* ||C10592|
|CellLight® Golgi-RFP *BacMam 2.0* ||C10593|
|CellLight® Lysosomes-GFP *BacMam 1.0* ||C10596|
|CellLight® Lysosomes-RFP *BacMam 2.0* ||C10597|
|CellLight® Mitochondria-GFP *BacMam 2.0* ||C10600|
|CellLight® Mitochondria-RFP *BacMam 2.0* ||C10601|
|CellLight® Peroxisomes-GFP *BacMam 2.0* ||C10604 |