Enhance your research with Invitrogen's cell biology publications—BioProbes® , ProbesOnline™, and BioPath Online™.
Pathway Focus: Metabolic Disorders
Metabolic syndrome is a combination of medical disorders that increase the risk of developing cardiovascular disease and diabetes. Serum amyloid A (SAA), c-reactive protein (CRP), and plasminogen activator inhibitor-1 (PAI-1) play important roles in these diseases. SAA and CRP are acute-phase proteins with plasma concentrations that increase in response to inflammation; inflammatory cells secrete a variety of cytokines into the bloodstream, including IL-6 and TNFα.
The liver responds by producing a large number of these acute-phase reactants. One type of acute-phase protein is plasminogen, which functions to degrade blood clots (fibrinolysis). PAI-1 inhibits the fibrinolysis process.
We offer sandwich ELISA kits to quickly detect and quantify specific proteins to support your normal and diseased model research. The ELISA kits allow results to be collected in an easy and reproducible fashion. Calibrated standard curves are provided to help accurately quantify the level of protein in each experimental run. ELISA technology allows a more detailed understanding of protein levels in metabolic disorders.
The Amplex® Red Cholesterol Assay Kit provides a very simple and sensitive fluorometric method to permit accurate quantitation of cholesterol in research samples using a fluorescence microplate reader or fluorometer. Because a large portion of cholesterol in blood is in the form of cholesteryl esters, the assay is based on an enzyme-coupled reaction that detects both free cholesterol and cholesteryl esters.
Cholesteryl esters are hydrolyzed by cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase to yield H2O2 and the corresponding ketone product. The H2O2 is then detected using Amplex® Red reagent, a highly sensitive and stable probe for H2O2. In the presence of horseradish peroxidase (HRP), Amplex® Red reagent reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent resorufin. Because resorufin has absorption/emission maxima of ~571/585 nm, there is little interference from autofluorescence in most biological samples.
Akt/protein kinase B (Akt) and its signaling cascade are involved in a number of different cellular functions, including glucose uptake, glycogen synthesis, cell growth, survival, apoptosis, protein synthesis, and endothelial nitric oxide production. In Type 1 diabetes, the kidney is exposed to fluctuating levels of blood glucose and exogenous insulin, which is administered as the major treatment of the disease. It has been well established that both glucose and insulin can modulate Akt activity in various cell types and tissues, and thus can affect the signaling cascade and subsequent cellular processes.
We offer pan and phosphorylation site–specific antibodies that can be used in research to check the activation status of Akt, mTOR, 4E-BP1, and other targets in the signaling cascade. ABfinity™ antibodies are rabbit recombinant antibodies produced by cloning the protein-specific genes and expressing them in mammalian cells. The result is a highly specific antibody with consistent lot-to-lot performance. New ABfinity™ antibodies include an antibody against Akt that is phosphorylated at serine 473, as well as antibodies against RANBP3,CD117 (c-Kit)[pY703], MEK2, SAA1, and Hexokinase 2, which are all involved in Akt or related pathways.
|Product||Applications ||Clone||Reactive Species||Cat No. |
|RANBP3 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified ||WB, ICC/IF ||B24H15L41||Human||700076|
|CD117 (c-Kit)[pY703]ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified ||WB ||53H8L29||Human||700135|
|MEK2 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified ||WB, ICC/IF ||B19H36L8||Human||700829 |
|SAA1 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified ||WB ||D9H4L41||Mouse||700830|
|Hexokinase 2 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified ||WB ||2H8L6||Human||700422 |
|AKT [pS473] ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified ||ELISA ||99H9L9||Human||44621G|
ICC = immunocytochemistry. IF = immunofluorescence. WB = western blot.