|October 2010 |
BenchTopics™ Newsletter for Protein Technologies and Advanced Cloning
Don’t miss the online in vivo RNAi delivery seminar
RNAi is a powerful tool that can be used in the drug discovery process to identify new targets for drug design or to validate the target for which a drug was designed. Unfortunately, delivering RNAi duplexes to the appropriate tissue remains a major bottleneck for the development of in vivo RNAi. In this seminar, you will learn techniques and considerations for successful in vivo RNAi experiments and will get to hear how our new a new, commercially available, in vivo delivery reagent, Invivofectamine™ 2.0, helps overcome these challenges.
A Life Technologies scientist will host the online seminar, so you can attend from the comfort of your own desk.
AcTEV™ Protease for efficient cleavage and removal of epitope tags and fusion partners
Use AcTEV™ Protease, an enhanced version of tobacco etch virus (TEV) protease, to efficiently cleave and remove solubility, secretion, detection, and purification tags from recombinant proteins. AcTEV™ Protease has the same sequence and cleavage site-specificity as TEV (Glu-Asn-Leu-Tyr-Phe-Gln v Gly). AcTEV™ Protease retains 100% activity, even after incubation for 1 week at 4°C. Under the same incubation conditions, non-enhanced TEV protease looses 25% of its activity within 24 hours (see figure).
To find all Invitrogen™ protein expression vectors containing a TEV cleavage site, browse to the Vector Selector Tool, and under “N-term Protease Cleavage” select TEV. For free, online primer design with a one-step addition of a TEV cleavage sequence to your gene, go to OligoPerfect™ Designer.
The NativePAGE™ Novex® Bis-Tris Gel system provides a sensitive and high-resolution method for the assessment of the purity of native proteins, the analysis of native membrane protein complexes, native soluble proteins, and molecular mass estimations. The NativePAGE™ Novex® Bis-Tris Gel system is a native (nondenaturing) precast polyacrylamide mini gel electrophoresis system that operates at a near-neutral pH of 7.5. This neutral environment protects both the proteins and the gel matrix during electrophoresis, providing for higher band resolution than the traditional Laemmli system. (Figure reproduced from Kubota S et al. (2006) Proc Natl Acad Sci USA 103:8360–8365.)
The binding of G-250 to proteins offers the following advantages resulting in high-resolution native electrophoresis (Schägger, 2001)
To see additional details and to order see
ProtoArray® human protein microarrays continue to help in discovery of new disease markers
Auto-antibodies are ideal molecules for establishing new diagnostic and efficacy biomarkers. The ProtoArray® human microarray offers a printed surface containing over 9,000 full-length human antigens, representing a wide spectrum of potential auto-antigens from a variety of protein classes. The ProtoArray® microarray’s success in identifying auto-antibody–based biomarkers was recently demonstrated in a publication by Cell Gensys Inc. The researchers examined changes in patient immune profiles on the array before and after immunotherapy in prostate cancer patients. The researchers identified several auto-antibodies that were elevated in response to the treatment that may serve as viable biomarkers for positive therapy response in larger patient samples.
To find out more about ProtoArray® microarrays and biomarker identification, visit us online at www.invitrogen.com/absoluteid. We offer catalog ProtoArray® microarrays for purchase, and we can also create custom microarrays in our full-service laboratory to meet your experimental needs.
Life Technologies™ is proud to announce the launch of a new set of cloning tools that will revolutionize the way you perform research. With the addition of these products to our market-leading cloning tools portfolio, you’ll be able to access novel and innovative cloning platforms and mutagenesis solutions in your laboratory. These new cloning tools offer significant advantages in everything from constructing simple DNA vectors to assembling complex pathways or mini-chromosomes:
• Easy and Powerful — Clone up to 10 DNA fragments, with the sequence of your choice, simultaneously in a single vector (up to 110 Kbp); no restriction digestion, ligation or recombination sites required
• Flexibility — Use our linear vectors or a vector of your choice, clone pre-existing DNA fragments that have no end-homology without further modifications
• Precision and Efficiency —Designed to let you clone what you want, where you want, in the orientation you want, and achieve up to 90% correct clones with no extra sequences left behind
Also available is the new DNA Oligo Designer, an intuitive, web-based tool to guide your design. The tool minimizes the planning time required for designing the PCR primers, creates the end-terminal homology required for seamless assembly,
identifies potential pitfalls linked to your specific sequences, and performs in silico cloning using your sequences. The DNA Oligo Designer also provides you with a graphic representation of the final assembled molecule as well as a downloadable GenBank file compatible with VectorNTI® software.
Learn more about these new tools today!
Ambion® In Vivo siRNA
Ambion® In Vivo siRNA molecules are chemi¬cally modified, 21-mer double-stranded siRNAs that are recognized by the RNA-induced silencing complex (RISC) to mediate inhibition of a target gene. Proprietary chemical modifications allow Ambion® In Vivo siRNAs to overcome many in vivo–specific obstacles, ensuring their effectiveness and stability. Ambion® In Vivo siRNAs are at least one hundred times more stable in 90% mouse serum than unmodified siRNAs.
As the field of RNA interference (RNAi) matures, researchers are becoming increasingly interested in discovering the in vivo effects of gene silencing. However, due to the presence of high levels of nucleases in blood and other biological fluids, in vivo RNAi experiments are more challenging than those performed in vitro. This increased challenge necessitates siRNAs that are specifically developed for in vivo applications, incorporating optimal sequence design features, appropriate chemical modifications, and the use of high-quality materials. We are now able to offer the most advanced siRNAs for in vivo applications—Ambion® In Vivo siRNAs.
Ambion® in vivo siRNAs, the new standard for in vivo RNAi applications, offer:
Each month we feature a different Life Technologies employee and share his or her story. This month, we interviewed William Staerker, Global Market Development Manager, Molecular Biology Reagents.
October competition: Did you dress up for Halloween? Send us a picture, and we’ll send an iPod touch® device to the person wearing the best costume. To enter, simply send picture to firstname.lastname@example.org by November 5, 2010.* No purchase necessary to enter or win. To be eligible, you must be a legal U.S. (excluding Puerto Rico) or Canadian (excluding Quebec) resident, age 21 and older and employed in a capacity where you perform life sciences research in a private, educational, public, or government research facility or institution. To participate, you must submit picture of yourself in a Halloween costume to email@example.com, no later than November 5, 2010. Only one submission per person. The eligible entrant who submitted the best picture as solely determined by a panel of judges composed of Invitrogen’s employees (a “Winner”) will win an iPod® touch device. The decision of the panel is final. Void where prohibited, restricted, licensed or otherwise limited by law. The Winner must warrant that they are not prohibited by employment, contract, law or otherwise, including the policies and practices of their employer, from entering or accepting a prize from Invitrogen, the contest sponsor. The Winner will be notified on or about November 7, 2010 by certified mail, e-mail, or telephone. Apple, Inc. is not a sponsor of this contest.