Spectral characteristics of PeakFlow flow cytometry reference beads—Table 23.9

Cat # Size (µm) Nominal Color Abs * (nm) Em * (nm) Emission Matches Cells Stained with:
P14825 2.5 Blue 400 † 460 DAPI, Hoechst dyes
P14826 6.0
P14827 2.5 Green 505 ‡ 515 Fluorescein, Alexa Fluor 488 dye, Oregon Green 488 dye, DiOC18(3) ("DiO")
P14828 6.0
P14829 2.5 Orange 570  575 R-phycoerythrin, tetramethylrhodamine, Alexa Fluor 568 dye, DiIC18(3) ("DiI")
P14830 6.0
P14831 2.5 Carmine 580  620 Propidium iodide, Texas Red dye, Alexa Fluor 594 dye
P14832 6.0
P24670 6.0 Claret 645 § 680 TOTO-3, Alexa Fluor 647 dye, Cy5 dye, DiIC18(5) ("DiD")
P24671 6.0 Ultra red 665 § 695 Alexa Fluor 660 dye, Cy5.5 dye
P24672 6.0 Infrared 735 770 Alexa Fluor 750 dye, Cy7 dye, DiIC18(7) ("DiR")
* Approximate absorption and emission maxima for beads in suspension. † Suitable for excitation by the UV (351–364 nm) spectral line of argon-ion lasers. ‡ Suitable for excitation by the 488 nm spectral line of argon-ion lasers. § Suitable for excitation by the 633 nm spectral line of He-Ne lasers.