Invitrogen Life Science > References > Molecular Probes: The Handbook > List of Tables > Reactive oxygen species—Table 18.1

Reactive oxygen species—Table 18.1

Reactive Oxygen Species	Structure	Detection Reagents
Hydrogen peroxide	H₂O₂	Carboxy-H₂DCFDA (C400) CM-H₂DCFDA (C6827) Dihydrocalcein AM (D23805) Dihydrorhodamine 123 (D632, D23806) Dihydrorhodamine 6G (D633) H₂DCFDA (D399) Lucigenin (L6868) Luminol (L8455) RedoxSensor Red CC-1 (R14060)
Hydroxyl radical *	HO·	3'-(p-Aminophenyl) fluorescein (APF, A36003) 3'-(p-Hydroxyphenyl) fluorescein (HPF, H36004) CM-H₂DCFDA (C6827) Proxyl fluorescamine (C7924) TEMPO-9-AC (A7923)
Hypochlorous acid	HOCl	Aminophenyl fluorescein (APF, A36003) Dihydrorhodamine 123 (D632, D23806) Luminol (L8455)
Nitric oxide	NO	DAF-FM (D23841) DAF-FM diacetate (D23842, D23844) DAA (D23840) 2,3-Diaminonaphthalene (D7918) Luminol (L8455)
Peroxyl radical, including both alkylperoxyl and hydroperoxyl radicals (wherein R = H)	ROO·	BODIPY FL EDA (D2390) BODIPY 665/676 (B3932) H₂DCFDA (D399) Carboxy-H₂DCFDA (C400) CM-H₂DCFDA (C6827) DPPP (D7894) Luminol (L8455) cis-Parinaric acid (P36005) RedoxSensor Red CC-1 (R14060)
Peroxynitrite anion †	ONOO^–	3'-(p-Aminophenyl) fluorescein (APF, A36003) 3'-(p-Hydroxyphenyl) fluorescein (HPF, H36004) H₂DCFDA (D399) Carboxy-H₂DCFDA (C400) CM-H₂DCFDA (C6827) Coelenterazine (C2944) Dihydrorhodamine 123 (D632, D23806) Dihydrorhodamine 6G (D633) Luminol (L8455)
Singlet oxygen ‡	¹O₂	Singlet Oxygen Sensor Green reagent (S36002) trans-1-(2'-methoxyvinyl)pyrene (M7913)
Superoxide anion	·O₂^–	Coelenterazine (C2944) Dihydroethidium (D1168, D11347, D23107) Fc OxyBURST Green assay reagent (F2902) OxyBURST Green H₂DCFDA SE (D2935) OxyBURST Green H₂HFF BSA (O13291) Lucigenin (L6868) Luminol (L8455) MCLA (M23800) MTT (M6494) NBT (N6495) RedoxSensor Red CC-1 (R14060) TEMPO-9-AC (A7923) XTT (X6493)
* Hydroxyl radicals can also be photosensitized by malachite green isothiocyanate (M689) or generated by a N-(1,10-phenanthrolin-5-yl)iodoacetamide (P6879) metal–ligand complex. † 3-Nitrotyrosine, a product of this potent nitrating reagent, can be detected with an anti-nitrotyrosine antibody (A21285). ‡ Singlet oxygen can also be photosensitized by hypericin (H7476), rose bengal diacetate (R14000) and merocyanine 540 (M24571).