Molecular Probes azide and alkyne derivatives—Table 3.1

Compound or Fluorophore	Azide or Alkyne	Cat. No.	Application
Azide- or Alkyne-Modified Biomolecules for Metabolic or Enzymatic Labeling
Click-iT AHA (L-azidohomoalanine)	azide	C10102	Monitor nascent protein synthesis or inhibition
Click-iT HPG (L-homopropargylglycine)	alkyne	C10186	Monitor nascent protein synthesis or inhibition
Click-iT farnesyl alcohol, azide	azide	C10248	Identification of isoprenylated proteins
Click-iT geranylgeranyl alcohol, azide	azide	C10249	Identification of isoprenylated proteins
Click-iT fucose alkyne (tetraacetylfucose alkyne)	alkyne	C10264	Identification of fucoslyated proteins
Click-iT palmitic acid, azide	azide	C10265	Identification of protein fatty acylation
Click-iT myristic acid, azide	azide	C10268	Identification of protein fatty acylation
Click-iT GalNAz (tetraacetylated N-azidoacetylgalactosamine)	azide	C33365	Identification of O-linked glycoproteins
Click-iT ManNAz (tetraacetylated N-azidoacetylmannosamine)	azide	C33366	Identification of sialic acid–modified glycoproteins
Click-iT GlcNAz (tetraacetylated N-azidoacetylglucosamine)	azide	C33367	Identification of O-linked glycoproteins
Azide- or Alkyne-Modified Fluorophores and Haptens for Detection (Ex/Em) *
Alexa Fluor 488 (495/519)	azide	A10266	Fluorescent dye or hapten †
	alkyne	A10267	Fluorescent dye or hapten †
	DIBO alkyne	C10405	Fluorescent dye (or hapten †) for copper-free labeling and detection of azide-modified targets
Alexa Fluor 555 (555/565)	azide	A20012	Fluorescent dye
	alkyne	A20013	Fluorescent dye
	DIBO alkyne	C10406	Fluorescent dye for copper-free labeling and detection of azide-modified targets
Alexa Fluor 594 (590/617)	azide	A10270	Fluorescent dye
	alkyne	A10275	Fluorescent dye
	DIBO alkyne	C10407	Fluorescent dye for copper-free labeling and detection of azide-modified targets
Alexa Fluor 647 (650/668)	azide	A10277	Fluorescent dye
	alkyne	A10278	Fluorescent dye
	DIBO alkyne	C10408	Fluorescent dye for copper-free labeling and detection of azide-modified targets
Biotin (NA)‡	azide	B10184	Hapten
	alkyne	B10185	Hapten
	DIBO alkyne	C10412	Hapten for copper-free labeling and detection of azide-modified targets
Oregon Green 488 (496/524)	azide	O10180	Fluorescent dye or hapten †
Oregon Green 488 (496/524)	alkyne	O10181	Fluorescent dye or hapten †
Tetramethylrhodamine (TAMRA) (555/580)	azide	T10182	Fluorescent dye or hapten †
	alkyne	T10183	Fluorescent dye or hapten †
	DIBO alkyne	C10410	Fluorescent dye for copper-free labeling and detection of azide-modified targets
* Absorption and fluorescence emission maxima, in nm. † As hapten, use with anti-dye antibody; see Anti-Dye and Anti-Hapten Antibodies—Section 7.4. ‡ Requires streptavidin for detection; see Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices—Section 7.6.