Fluorescent dyes successfully tested with the Image-iT FX signal enhancer—Table 7.8

Dyes * with potentially strong background fluorescence that is reduced with the Image-iT FX Signal Enhancer †
Fluorescein Atto 610 Alexa Fluor 594
Oregon Green 488 Cascade Blue Alexa Fluor 610
Oregon Green 514 Alexa Fluor 405 Alexa Fluor 633
Tetramethylrhodamine Alexa Fluor 430 Alexa Fluor 635
Texas Red Alexa Fluor 488 Alexa Fluor 647
Cascade Yellow Alexa Fluor 500 Alexa Fluor 660
Dy 565 Alexa Fluor 514 Alexa Fluor 680
Dy 630 Alexa Fluor 555 Alexa Fluor 700
Atto 590 Alexa Fluor 568 Alexa Fluor 750
Dyes * with potentially weak background fluorescence that is reduced with the Image-iT FX Signal Enhancer †
Cy5 Alexa Fluor 532  Allophycocyanin
Dy 635 Alexa Fluor 546  R-phycoerythrin
Marina Blue    
Background-free fluorescent dyes * that are not affected by the Image-iT FX Signal Enhancer ‡
Alexa Fluor 350 Pacific Blue Texas Red-X
IRTM 790 Rhodamine B Dy 550
Cy3 Rhodamine Red-X Dy 610
* All dyes were conjugated to streptavidin and tested at 10 µg/mL. † Background staining was blocked by the Image-iT FX signal enhancer. ‡ Staining was unaffected by the Image-iT FX signal enhancer.