Fluorescent dyes successfully tested with the Image-iT FX signal enhancer—Table 7.8
| Dyes * with potentially strong background fluorescence that is reduced with the Image-iT FX Signal Enhancer † | ||
|---|---|---|
| Fluorescein | Atto 610 | Alexa Fluor 594 |
| Oregon Green 488 | Cascade Blue | Alexa Fluor 610 |
| Oregon Green 514 | Alexa Fluor 405 | Alexa Fluor 633 |
| Tetramethylrhodamine | Alexa Fluor 430 | Alexa Fluor 635 |
| Texas Red | Alexa Fluor 488 | Alexa Fluor 647 |
| Cascade Yellow | Alexa Fluor 500 | Alexa Fluor 660 |
| Dy 565 | Alexa Fluor 514 | Alexa Fluor 680 |
| Dy 630 | Alexa Fluor 555 | Alexa Fluor 700 |
| Atto 590 | Alexa Fluor 568 | Alexa Fluor 750 |
| Dyes * with potentially weak background fluorescence that is reduced with the Image-iT FX Signal Enhancer † | ||
| Cy5 | Alexa Fluor 532 | Allophycocyanin |
| Dy 635 | Alexa Fluor 546 | R-phycoerythrin |
| Marina Blue | ||
| Background-free fluorescent dyes * that are not affected by the Image-iT FX Signal Enhancer ‡ | ||
| Alexa Fluor 350 | Pacific Blue | Texas Red-X |
| IRTM 790 | Rhodamine B | Dy 550 |
| Cy3 | Rhodamine Red-X | Dy 610 |
| * All dyes were conjugated to streptavidin and tested at 10 µg/mL. † Background staining was blocked by the Image-iT FX signal enhancer. ‡ Staining was unaffected by the Image-iT FX signal enhancer. | ||