CellLight reagents and their targeting sequences—Table 11.1
| Target | Targeting Sequence | Ref | GFP (489/508 nm) * | RFP (555/584 nm) * | Handbook Section |
|---|---|---|---|---|---|
| Actin | Human actin | 1 | C10582 | C10583 C10127 ‡ | Section 11.1 |
| Tubulin | Human tubulin | 2 | C10613 | C10614 | Section 11.2 |
| MAP4 | MAP4 | 3 | C10598 | C10599 | Section 11.2 |
| Talin | Human talin 2341–2541 | 4 | C10611 | C10612 | Section 11.2 |
| Chromatin | Histone 2B (H2B) | 5 | C10594 | C10595 | Section 12.5 |
| Mitochondria | Leader sequence of E1α pyruvate dehydrogenase | 6 | C10600 | C10601 | Section 12.2 |
| Lysosomes | Lamp1 (lysosomal-associated membrane protein 1) | 7 | C10596 | C10597 | Section 12.3 |
| Peroxisomes | Peroxisomal C-terminal targeting sequence | 8 | C10604 | Section 12.3 | |
| Endosomes | Rab5a | 9 | C10586 | C10587 | Section 12.3, Section 16.1 |
| Synaptosomes | Synaptophysin | 10 | C10609 | C10610 | Section 16.1 |
| Endoplasmic reticulum | ER signal sequence of calreticulin and KDEL (ER retention signal) | 11 | C10590 | C10591 | Section 12.4 |
| Golgi apparatus | Human golgi-resident enzyme N-acetylgalactosaminyltransferase 2 | 12 | C10592 | C10593 | Section 12.4 |
| Nucleus | LSV40 nuclear localization sequence | 13 | C10602 | C10603 | Section 12.5 |
| Plasma membrane | Myristoylation/palmitoylation sequence from Lck tyrosine kinase | 14 | C10607 † | C10608 † | Section 14.4 |
| Cytoplasm | No targeting sequence | B10383 | Section 14.7 | ||
* Approximate absorption (Abs) and fluorescence (Em) maxima, in nm; GFP (Green Fluorescent Protein) and RFP  (Red Fluorescent Protein) can be imaged using optical filters for fluorescein (FITC) and tetramethylrhodamine (TRITC) dyes, respectively. † Also available is CellLight Plasma Membrane-CFP (C10606), which generates a cyan-autofluorescent protein fused to the plasma membrane targeting sequence from Lck tyrosine kinase. ‡ This Cellular Lights Actin-RFP (C10127) contains the BacMam 1.0 vector, whereas all of the CellLight reagents listed in this table contain the BacMam 2.0 vector. 1. Curr Biol (1997) 7:176; 2. PLoS One (2009) 4:e8171; 3. J Cell Biol (1995) 130:639; 4. Plant J (2003) 33:775; 5. Curr Biol (1998) 8:377; 6. J Biol Chem (2004) 279:13044; 7. J Cell Sci (2005) 118:5243; 8. J Cell Biol (1989) 108:1657; 9. J Biol Chem (2009) 284:29218; 10. J Neurosci (2006) 26:3604; 11. FEBS Lett (1997) 405:18; 12. J Cell Biol (1998) 143:1505; 13. Trends Biochem Sci (1991) 16:478; 14. EMBO J (1997) 16:4983. | |||||
