Biotinylation and desthiobiotinylation reagents—Table 4.1

Reactive Moiety Biotin Derivative (Cat #)DSB-X Biotin Derivative (Cat #)
Aliphatic amine A1593, A1594, B1596 (XX), N6356 
AlkyneB10185 
AzideB10184 
Carboxylic acid B1595, B20656, B1592D20657
DNP-X–biocytin-X, SE B2604, F6348 (F) 
Hydrazide B1603, B2600 (XX)D20653 (X)
Hydroxylamine A10550 
Iodoacetamide B1591D30753
Maleimide M1602 
Succinimidyl ester (SE) B1513, B1582 (X), B1606 (XX), B6353 (SSE)(X), B6352 (SSE)(XX), A10495 (APEX), B30010 (Micro), B30756 (Micro), F2610 (F), F6347 (F)D20655 (D)
TS-Link thiosulfate T30754
(APEX) = APEX Antibody Labeling Kit. (D) = DSB-X Biotin Protein Labeling Kit. (F) = FluoReporter Protein Labeling Kit. (Micro) = Biotin-XX Microscale Protein Labeling Kit. (SE) = Succinimidyl ester. (SSE) = Sulfosuccinimidyl ester. (X) = Aminohexanoyl (7-atom) spacer separating the biotin or desthiobiotin and the reactive moiety. (XX) = Aminohexanoylaminohexanoyl (14-atom) spacer separating the biotin and the reactive moiety.