Amine-reactive xanthene derivatives in this section—Table 1.8
|Fluorophore (Abs/Em) *||COOH||Succinimidyl Ester||Other||Protein and Nucleic Acid Labeling Kits||Notes|
|Eosin (524/544)||E18 (ITC)5|
|Fluorescein (494/518)||C1359 5 |
|C2210 5 |
|F143 (ITC)5 |
|F6433 (F) |
|JOE (520/548)||C6171MP 6|
|Oregon Green 488 (496/524)||O6146 5||O6147 5 |
|O6080 M||F6153 (F) |
|Oregon Green 514 (511/530)||O6138 M||O6139 6|
|Rhodamine Green (502/527)||R6107 M |
|* The numbers in parentheses reflect the absorption (Abs) and fluorescence emission (Em) maxima, in nm, of the goat anti–mouse IgG antibody or dextran conjugates in aqueous buffer.|
Superscripts 5, 6 and M correspond to 5-isomer, 6-isomer and a mixture of 5- and 6-isomers, respectively.
(APEX) = APEX Antibody Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2).
(C) = Caged; the probe is nonfluorescent until the caging group is removed by UV illumination.
(COOH) = Carboxylic acid.
(DTA) = Dichlorotriazine.
(EX) = A seven-atom spacer that is more hydrophilic than X.
(F) = FluoReporter Protein Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2).
(ITC) = Isothiocyanate.
(P) = Easy-to-Use Protein Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2).
(U) = ULYSIS Nucleic Acid Labeling Kit (Labeling Oligonucleotides and Nucleic Acids—Section 8.2).
(X) = Aminohexanoyl spacer separating the dye and SE.
(Z) = Zenon Antibody Labeling Kit (Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3).