Alexa Fluor active esters and kits for labeling proteins and nucleic acids—Table 1.4
| Alexa Fluor Dye | Fluorescence Color (Abs/Em) * | Succinimidyl Ester, TFP Ester or SDP Ester | Kits for Labeling Proteins | Kits for Labeling Nucleic Acids and Oligonucleotides | |||
|---|---|---|---|---|---|---|---|
| Protein Labeling Kits | Antibody Labeling Kits | Zenon Antibody Labeling Kits | |||||
| Alexa Fluor 350 | Blue (346/442) | A10168 † | A10170 (P) | A20180 (Mab) | Z25000 (M IgG1) Z25100 (M IgG2a) Z25200 (M IgG2b) Z25300 (R IgG) Z25400 (H IgG) | ||
| Alexa Fluor 405 | Blue (402/421) | A30000 ‡ A30100 † | Z25013 (M IgG1) Z25113 (M IgG2a) Z25213 (M IgG2b) Z25313 (R IgG) | ||||
| Alexa Fluor 430 | Yellow-green (434/539) | A10169 † | A10171 (P) | Z25001 (M IgG1) Z25301 (R IgG) | |||
| Alexa Fluor 488 | Green (495/519) | A20000 ‡ § A20100 † § A30005 (TFP) A30052 (SDP) | A10235 (P) ** A30006 (Micro) ** | A10468 (APEX) ** A20181 (Mab) ** | Z25002 (M IgG1) Z25090 TSA) (M IgG1) Z25102 (M IgG2a) Z25202 (M IgG2b) Z25302 (R IgG) Z25602 (G IgG) Z25402 (H IgG) | U21650 (ULYSIS) A21665 (ARES) F32947 (FT) F32952 (FT) A20191 (Oligo) | |
| Alexa Fluor 514 | Yellow-green (518/540) | A30002 ‡ § | |||||
| Alexa Fluor 532 | Yellow (531/554) | A20001 ‡ A20101MP † | A10236 (P) | A20182 (Mab) | Z25003 (M IgG1) Z25303 (R IgG) | U21651 (ULYSIS) | |
| Alexa Fluor 546 | Orange (556/573) | A20002 ‡ § A20102 † § | A10237 (P) | A20183 (Mab) | Z25004 (M IgG1) Z25104 (M IgG2a) Z25204 (M IgG2b) Z25304 (R IgG) | U21652 (ULYSIS) A21667 (ARES) | |
| Alexa Fluor 555 | Red-orange (555/565) | A20009 ‡ A20109 † | A20174 (P) A30007 (Micro) | A10470 (APEX) A20187 (Mab) | Z25005 (M IgG1) Z25105 (M IgG2a) Z25205 (M IgG2b) Z25305 (R IgG) Z25605 (G IgG) Z25405 (H IgG) | A21677 (ARES) F32948 (FT) F32953 (FT) | |
| Alexa Fluor 568 | Red-orange (578/603) | A20003 ‡ § A20103 † § | A10238 (P) | A10494 (APEX) A20184 (Mab) | Z25006 (M IgG1) Z25106 (M IgG2a) Z25206 (M IgG2b) Z25306 (R IgG) Z25606 (G IgG) | U21653 (ULYSIS) | |
| Alexa Fluor 594 | Red (590/617) | A20004 ‡ § A20104 † § | A10239 (P) A30008 (Micro) | A10474 (APEX) A20185 (Mab) | Z25007 (M IgG1) Z25107 (M IgG2a) Z25207 (M IgG2b) Z25307 (R IgG) Z25607 (G IgG) Z25407 (H IgG) | U21654 (ULYSIS) A21669 (ARES) F32949 (FT) F32954 (FT) | |
| Alexa Fluor 610 | Red (612/628) | A30050 (X) ‡ | |||||
| Alexa Fluor 633 †† | Far-red (632/647) | A20005 ‡ § A20105 † § | A20170 (P) | ||||
| Alexa Fluor 647 †† | Far-red (650/668) | A20006 ‡ A20106 † | A20173 (P) A30009 (Micro) | A10475 (APEX) A20186 (Mab) S30044 (SAIVI) | Z25008 (M IgG1) Z25108 (M IgG2a) Z25208 (M IgG2b) Z25308 (R IgG) Z25608 (G IgG) Z25408 (H IgG) | U21660 (ULYSIS) A21676 (ARES) F32950 (FT) F32955 (FT) A20196 (Oligo) | |
| Alexa Fluor 660 †† | Near-infrared (663/690) | A20007 ‡ | A20171 (P) | Z25009 (M IgG1) | U21656 (ULYSIS) A21671 (ARES) | ||
| Alexa Fluor 680 †† | Near-infrared (679/702) | A20008 ‡ A20108 † | A20172 (P) | S30045 (SAIVI) | Z25010 (M IgG1) Z25110 (M IgG2a) Z25210 (M IgG2b) Z25310 (R IgG) | ||
| Alexa Fluor 700 †† | Near-infrared (702/723) | A20010 ‡ A20110 † | Z25011 (M IgG1) | ||||
| Alexa Fluor 750 †† | Near-infrared (749/775) | A20011 ‡ A20111 † | S30046 (SAIVI) | Z25312 (R IgG) | |||
| Alexa Fluor 790 †† | Near-infrared (782/805) | A30051 | |||||
| * Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † 5 mg unit size. ‡ 1 mg unit size. § Mixed isomers. ** The APEX Alexa Fluor 488 Antibody Labeling Kits contain the Alexa Fluor 488 carboxylic acid SDP ester, whereas the other Alexa Fluor 488 protein labeling kits contain the amine-reactive Alexa Fluor 488 carboxylic acid TFP ester; the Alexa Fluor 488 kits for labeling nucleic acids and oligonucleotides contain the Alexa Fluor 488 succinimidyl ester. †† Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope. The Alexa Fluor Labeling Kits are described in detail in Kits for Labeling Proteins and Nucleic Acids—Section 1.2. (APEX) = APEX Antibody Labeling Kit. (ARES) = ARES DNA Labeling Kit. (FT) = FISH Tag DNA Kit or FISH Tag RNA Kit. (G IgG) = Zenon Goat IgG Labeling Kit. (H IgG) = Zenon Human IgG Labeling Kit. (M IgG) = Zenon Mouse IgG Labeling Kit. (Mab) = Monoclonal Antibody Labeling Kit. (Micro) = Alexa Fluor Microscale Protein Labeling Kit. (Oligo) = Alexa Fluor Oligonucleotide Amine Labeling Kit. (P) = Easy-to-Use Protein Labeling Kit. (R IgG) = Zenon Rabbit IgG Labeling Kit. (SAIVI) = SAIVI Antibody Labeling Kit. (SDP) = Sulfodichlorophenol ester. (TFP) = Tetrafluorophenyl ester. (TSA) = Enhanced with TSA technology. (ULYSIS) = ULYSIS Nucleic Acid Labeling Kit. (X) = An aminohexanoyl spacer between the dye and the SE. | |||||||
