Active esters and kits for labeling proteins and nucleic acids—Table 1.2

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Label Fluorescence Color (Abs/Em) * Succinimidyl Ester, TFP Ester or SDP Ester Protein Labeling Kits Zenon Antibody Labeling Kits Nucleic Acid Labeling Kits
Alexa Fluor 350 Blue
(346/442)		 A10168 A20180 (Mab)
A10170 (P)		 Z25000 (M IgG1)
Z25100 (M IgG2a)
Z25200 (M IgG2b)
Z25300 (R IgG)
Z25400 (H IgG)

  
Marina Blue Blue
(365/460)		 M10165      
Pacific Orange Orange
(400/551)		 P30253 P30014 (Mab)
P30016 (P)		 Z25256 (M IgG1)
Z25257 (M IgG2a)
  
Alexa Fluor 405 Blue
(402/421)		 A30000
A30100		   Z25013 (M IgG1)
Z25113 (M IgG2a)
Z25213 (M IgG2b)
Z25313 (R IgG)
  
Pacific Blue Blue
(410/455)		 P10163 A10478 (APEX)
P30013 (Mab)
P30012 (P)		 Z25041 (M IgG1)
Z25156 (M IgG2a)
Z25341 (R IgG)
  
Alexa Fluor 430 Yellow-green
(434/539)		 A10169 A10171 (P) Z25001 (M IgG1)
Z25301 (R IgG)
  
Fluorescein-EX Green
(494/518)		 F6130 F10240 (P)
F6433 (F)		 Z25042 (M IgG1)
Z25342 (R IgG)
  
FITC Green
(494/518)		   F6434 (F)    
Alexa Fluor 488 Green
(495/519)		 A20000 †
A20100 †
A30005 (TFP)
A30052 (SDP)		 A10468 ‡ (APEX)
A30006 ‡ (Micro)
A20181 ‡ (Mab)
A10235 ‡ (P)		 Z25002 (M IgG1)
Z25090 (TSA)(M IgG1)
Z25102 (M IgG2a)
Z25202 (M IgG2b)
Z25302 (R IgG)
Z25602 (G IgG)
Z25402 (H IgG)
 U21650 (ULYSIS)
A21665 (ARES)
F32947 (FT)
F32952 (FT)
A20191 (Oligo)
Oregon Green 488 Green
(496/524)		 O6147
O6149		 A10476 (APEX)
O10241 (P)
F6153 (F) 		  U21659 (ULYSIS)
Oregon Green 514 Green
(511/530)		 O6139      
Alexa Fluor 514 Yellow-green
(518/540)		 A30002 †      
Alexa Fluor 532 Yellow
(531/554)		 A20001
A20101MP		 A20182 (Mab)
A10236 (P)		 Z25003 (M IgG1)
Z25303 (R IgG)
 U21651 (ULYSIS)
Alexa Fluor 546 Orange
(556/573)		 A20002 †
A20102 †		 A20183 (Mab)
A10237 (P)		 Z25004 (M IgG1)
Z25104 (M IgG2a)
Z25204 (M IgG2b)
Z25304 (R IgG)
 U21652 (ULYSIS)
A21667 (ARES)
Alexa Fluor 555 Red-orange (555/565) A20009
A20109		 A10470 (APEX)
A30007 (Micro)
A20187 (Mab)
A20174 (P)		 Z25005 (M IgG1)
Z25105 (M IgG2a)
z25205 (M IgG2b)
Z25305 (R IgG)
Z25605 (G IgG)
Z25405 (H IgG)
 A21677 (ARES)
F32948 (FT)
F32953 (FT)
Tetramethylrhodamine Red-orange (555/580) C2211
C6123
C1171 †
T6105 † (X)		      
Rhodamine Red Red-orange (570/590) R6160 (X) F6161 (F)    
Alexa Fluor 568 Red-orange (578/603) A20003 †
A20103 †		 A10494 (APEX)
A20184 (Mab)
A10238 (P)		 Z25006 (M IgG1)
Z25106 (M IgG2a)
Z25206 (M IgG2b)
Z25306 (R IgG)
Z25606 (G IgG)

 U21653 (ULYSIS)
Alexa Fluor 594 Red
(590/617)		 A20004 †
A20104 †		 A10474 (APEX)
A30008 (Micro)
A20185 (Mab)
A10239 (P)		 Z25007 (M IgG1)
Z25107 (M IgG2a)
Z25207 (M IgG2b)
Z25307 (R IgG)
Z25607 (G IgG)
Z25407 (H IgG)

 U21654 (ULYSIS)
A21669 (ARES)
F32949 (FT)
F32954 (FT)
Texas Red Red (595/615) T6134 † (X)
T20175 (X)		 T10244 (P)
F6162 (F)		 Z25045 (M IgG1)  
Alexa Fluor 610 Red
(612/628)		 A30050 (X)      
Alexa Fluor 633 § Far-red
(632/647)		 A20005 †
A20105 †		 A20170 (P)    
Alexa Fluor 635 § Far-red
(633/647)		        
Alexa Fluor 647 § Far-red
(650/668)		 A20006
A20106		 A10475 (APEX)
A30009 (Micro)
A20186 (Mab)
S30044 (SAIVI)
A20173 (P)		 Z25008 (M IgG1)
Z25108 (M IgG2a)
Z25208 (M IgG2b)
Z25308 (R IgG)
Z25608 (G IgG)
Z25408 (H IgG)
 U21660 (ULYSIS)
A21676 (ARES)
F32950 (FT)
F32955 (FT)
A20196 (Oligo)
Alexa Fluor 660 § Near-infrared
(663/690)		 A20007 A20171 (P) Z25009 (M IgG1)  
Alexa Fluor 680 § Near-infrared (679/702) A20008
A20108		 S30045 (SAIVI)
A20172 (P)		 Z25010 (M IgG1)
Z25110 (M IgG2a)
Z25210 (M IgG2b)
Z25310 (R IgG)		  
Alexa Fluor 700 § Near-infrared
(702/723)		 A20010
A20110		   Z25011 (M IgG1)  
Alexa Fluor 750 § Near-infrared (749/775) A20011
A20111		 S30046 (SAIVI) Z25312 (R IgG)  
Alexa Fluor 790 §Near-infrared (782/805)A30051   
Biotin NA B1606 (XX)
B6353 (X)		 A10495 (APEX)
B30010 (Micro)
B30756 (Micro)
F2610 (FB)
F6347 (FMB)		 Z25052 (M IgG1)
Z25152 (M IgG2a)
Z25252 (M IgG2b)
Z25352 (R IgG)
Z25452 (H IgG)		  
DNP–biotin NA (364/none) B2604 (X) F6348 (F)    
DSB-X biotin NA   D20655 (D)    
* Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ These Alexa Fluor 488 protein labeling kits contain either the amine-reactive Alexa Fluor 488 carboxylic acid 5-TFP ester (Micro, Mab, P) or the amine-reactive Alexa Fluor 488 carboxylic acid 5-SDP ester (APEX); whereas the Alexa Fluor 488 nucleic acid labeling kits contain the Alexa Fluor 488 carboxylic acid succinimidyl ester. § Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope. NA = Not applicable.

(APEX) = APEX Antibody Labeling Kit.
(ARES) = ARES DNA Labeling Kit.
(D) = DSB-X Biotin Protein Labeling Kit.
(F) = FluoReporter Protein Labeling Kit.
(FB) = FluoReporter Biotin-XX Protein Labeling Kit.
(FMB) = FluoReporter Mini-Biotin-XX Protein Labeling Kit.
(FT) = FISH Tag DNA Kit or FISH Tag RNA Kit.
(G IgG) = Zenon Goat IgG Labeling Kit.
(H IgG) = Zenon Human IgG Labeling Kit.
(M IgG) = Zenon Mouse IgG Labeling Kit.
(Mab) = Monoclonal Antibody Labeling Kit.
(Micro) = Alexa Fluor Microscale Protein Labeling Kit or Biotin-XX Microscale Protein Labeling Kit.
(Oligo) = Alexa Fluor Oligonucleotide Amine Labeling Kit.
(P) = Easy-to-Use Protein Labeling Kit.
(R IgG) = Zenon Rabbit IgG Labeling Kit.
(SAIVI) = SAIVI Antibody Labeling Kit.
(SDP) = Sulfodichlorophenol ester.
(TFP) = Tetrafluorophenyl ester.
(TSA) = Enhanced with TSA technology.
(ULYSIS) = ULYSIS Nucleic Acid Labeling Kit.
(X) = An aminohexanoyl spacer between the dye and the SE.