Seminal Articles Using Molecular Probes Luminescence Based Protein Detection Technologies - Note 9.1
"Detection technologies in proteome analysis." W.F. Patton, J Chromatogr B Analyt Technol Biomed Life Sci 771, 3 (2002). "Making blind robots see: The synergy between fluorescent dyes and imaging devices in automated proteomics." W.F. Patton, Biotechniques 28, 944 (2000). "A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics." W.F. Patton, Electrophoresis 21, 1123 (2000). "Better approaches to finding the needle in a haystack: Optimizing proteome analysis through automation." M.F. Lopez, Electrophoresis 21, 1082 (2000). |
NanoOrange Protein Quantitation Assay
"Development and characterization of the NanoOrange protein quantitation assay: A fluorescence-based assay of proteins in solution." L.J. Jones, R.P. Haugland, V.L Singer, Biotechniques 34, 850 (2003).
Pro-Q Diamond Phosphoprotein Gel and Blot Stains
"Strategies and solid-phase formats for the analysis of protein and peptide phosphorylation employing a novel fluorescent phosphorylation sensor dye." K. Martin et al., Comb Chem High Throughput Screen 6, 331 (2003).
"Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye." B. Schulenberg et al., J Biol Chem 278, 27251 (2003).
"Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye." K. Martin et al., Proteomics 3, 1244 (2003).
"Global quantitative phosphoprotein analysis using multiplexed proteomics technology." T.H. Steinberg et al., Proteomics 3, 1128 (2003).
Pro-Q Emerald Glycoprotein Gel and Blot Stains
"Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots." T.H. Steinberg et al., Proteomics 1, 841 (2001).
Pro-Q Sapphire Oligohistidine Gel Stains
"Fluorescence detection and quantitation of recombinant proteins containing oligohistidine tag sequences directly in sodium dodecyl sulfate–polyacrylamide gels." C. Hart et al., Electrophoresis 24, 599 (2003).
SYPRO Ruby Protein Gel Stain
"Quantitative evaluation of proteins in one- and two-dimensional polyacrylamide gels using a fluorescent stain." J.C. Nishihara and K.M Champion, Electrophoresis 23, 2203 (2002).
"A novel subfractionation approach for mitochondrial proteins, a three-dimensional mitochondrial proteome map." B.J. Hanson et al., Electrophoresis 22, 950 (2001).
"Mass spectrometry compatibility of two-dimensional gel protein stains." W.M. Lauber et al., Electrophoresis 22, 906 (2001).
"Comparison of three different fluorescent visualization strategies for detecting Escherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresis." K.N. Berggren et al., Proteomics 1, 54 (2001).
"A comparison of silver stain and SYPRO Ruby protein gel stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling." M.F. Lopez et al., Electrophoresis 21, 3673 (2000).
"Postelectrophoretic staining of proteins separated by two-dimensional gel electrophoresis using SYPRO dyes." J.X. Yan et al., Electrophoresis 21, 3657 (2000).
"Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex." K. Berggren et al., Electrophoresis 21, 2509 (2000).
"The current state of two-dimensional electrophoresis with immobilized pH gradients." A. Görg et al., Electrophoresis 21, 1037 (2000).
SYPRO Ruby and SYPRO Rose Plus Protein Blot Stains
"A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports." K. Berggren et al., Anal Biochem 276, 129 (1999).
"Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress." K. Kokame et al., J Biol Chem 275, 32846 (2000).
"An improved, luminescent europium-based stain for detection of electroblotted proteins on nitrocellulose or polyvinylidene difluoride membranes." C. Kemper et al., Electrophoresis 22, 881 (2001).
SYPRO Orange and SYPRO Red Protein Gel Stains
"Analysis of tear protein patterns of dry-eye patients using fluorescent staining dyes and two-dimensional quantification algorithms." F.H. Grus, P. Sabuncuo, A.J. Augustin, Electrophoresis 22, 1845 (2001).
"Green-light transilluminator for the detection without photodamage of proteins and DNA labeled with different fluorescent dyes." F.J. Alba, A. Bermudez, J.R. Daban, Electrophoresis 22, 399 (2001).
"Optimal filter combinations for photographing SYPRO Orange or SYPRO Red dye-stained gels." T.H. Steinberg, H.M. White, V.L. Singer, Anal Biochem 248, 168 (1997).
"Applications of SYPRO Orange and SYPRO Red protein gel stains." T.H. Steinberg, R.P. Haugland, V.L. Singer, Anal Biochem 239, 238 (1996).
"SYPRO Orange and SYPRO Red protein gel stains: One-step fluorescent staining of denaturing gels for detection of nanogram levels of protein." T.H. Steinberg et al., Anal Biochem 239, 223 (1996).
SYPRO Tangerine Protein Gel Stain
"Simultaneous, two-color fluorescence detection of total protein profiles and β-glucuronidase activity in polyacrylamide gel." C. Kemper et al., Electrophoresis 22, 970 (2001).
"Fluorescence detection of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, nonfixative, saline solution." T.H. Steinberg et al., Electrophoresis 21, 497 (2000).
DyeChrome Western Blot Stain Kits
"A novel subfractionation approach for mitochondrial proteins: A three-dimensional mitochondrial proteome map." B.J. Hanson et al., Electrophoresis 22, 950 (2001).
"Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes." K.P. Top et al., Electrophoresis 22, 896 (2001).
Rhinohide Polyacrylamide Gel Strengthener
"An improved mechanically durable electrophoresis gel matrix that is fully compatible with fluorescence-based protein detection technologies." B. Schulenberg, B. Arnold, W.F. Patton. Proteomics 3, 1196 (2003).
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