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The Molecular Probes® Handbook
  • Fluorescence Fundamentals
  • Fluorophores and Their Amine-Reactive Derivatives—Chapter 1
  • Thiol-Reactive Probes—Chapter 2
  • Click Chemistry and Other Functional Group Modifications—Chapter 3
  • Biotin and Hapten Derivatives—Chapter 4
  • Crosslinking and Photoactivatable Reagents—Chapter 5
  • Ultrasensitive Detection Technology—Chapter 6
  • Antibodies, Avidins and Lectins—Chapter 7
  • Nucleic Acid Detection and Analysis—Chapter 8
  • Protein Detection and Analysis—Chapter 9
  • Enzyme Substrates and Assays—Chapter 10
  • Probes for Cytoskeletal Proteins—Chapter 11
  • Probes for Organelles—Chapter 12
  • Probes for Lipids and Membranes—Chapter 13
  • Fluorescent Tracers of Cell Morphology and Fluid Flow—Chapter 14
  • Assays for Cell Viability, Proliferation and Function—Chapter 15
  • Probes for Endocytosis, Receptors and Ion Channels—Chapter 16
  • Probes for Signal Transduction—Chapter 17
  • Probes for Reactive Oxygen Species, Including Nitric Oxide—Chapter 18
  • Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19
  • pH Indicators—Chapter 20
  • Indicators for Na+, K+, Cl– and Miscellaneous Ions—Chapter 21
  • Probes for Membrane Potential—Chapter 22
  • Antifades and Other Tools for Fluorescence Applications—Chapter 23
  • List of Tables
  • Technical Notes and Product Highlights
    • Definitions of Data Table Contents
    • The Alexa Fluor Dye Series—Note 1.1
    • Fluorescence Resonance Energy Transfer FRET—Note 1.2
    • Fluorescence Correlation Spectroscopy (FCS)—Note 1.3
    • Fluorescence Polarization (FP)—Note 1.4
    • Fluorescent Probes for Two-Photon Microscopy—Note 1.5
    • Antibody Structure and Classification—Note 7.1
    • MHC Tetramer Technology—Note 7.2
    • SYBR Safe DNA Gel Stain—Note 8.1
    • Monitoring Protein-Folding Processes with Environment-Sensitive Dyes—Note 9.1
    • Labeling Small Peptides with Amine-Reactive Dyes in Organic Solvents—Note 9.2
    • BacMam Gene Delivery and Expression Technology—Note 11.1
    • Using Organic Fluorescent Probes in Combination with GFP—Note 12.1
    • Mitochondria in Diseases—Note 12.2
    • Lipid-Mixing Assays of Membrane Fusion—Note 13.1
    • Antibodies for Detecting Membrane-Surface Labels—Note 13.2
    • Anti–Lucifer Yellow Dye, Anti–Alexa Fluor 405/Cascade Blue Dye and Anti–Alexa Fluor 488 Dye Antibodies—Note 14.1
    • Fluorescent Probes for Photoconversion of Diaminobenzidine Reagents—Note 14.2
    • Assays of Volume Change, Membrane Fusion and Membrane Permeability—Note 14.3
    • G Proteins and GTP Analogs for Binding Studies—Note 17.1
    • Loading and Calibration of Intracellular Ion Indicators—Note 19.1
    • MAXC Computer Program for Calculating Free Ca2+ Concentrations—Note 19.2
    • Using the Fluorescence SpectraViewer—Note 23.1
    • Selecting Optical Filters for Fluorescence Microscopy—Note 23.2

Technical Notes and Product Highlights

  • The Alexa Fluor Dye Series—Note 1.1
  • Fluorescence Resonance Energy Transfer (FRET)—Note 1.2
  • Fluorescence Correlation Spectroscopy (FCS)—Note 1.3
  • Fluorescence Polarization (FP)—Note 1.4
  • Fluorescent Probes for Two-Photon Microscopy—Note 1.5

  • Antibody Structure and Classification—Note 7.1
  • MHC Tetramer Technology—Note 7.2

  • SYBR Safe DNA Gel Stain—Note 8.1

  • Monitoring Protein-Folding Processes with Environment-Sensitive Dyes—Note 9.1
  • Labeling Small Peptides with Amine-Reactive Dyes in Organic Solvents—Note 9.2

  • BacMam Gene Delivery and Expression Technology—Note 11.1

  • Using Organic Fluorescent Probes in Combination with GFP—Note 12.1
  • Mitochondria in Diseases—Note 12.2

  • Lipid-Mixing Assays of Membrane Fusion—Note 13.1
  • Antibodies for Detecting Membrane-Surface Labels—Note 13.2

  • Anti–Lucifer Yellow Dye, Anti–Alexa Fluor 405/Cascade Blue Dye and Anti–Alexa Fluor 488 Dye Antibodies—Note 14.1
  • Fluorescent Probes for Photoconversion of Diaminobenzidine Reagents—Note 14.2
  • Assays of Volume Change, Membrane Fusion and Membrane Permeability—Note 14.3

  • G proteins and GTP Analogs for Binding Studies—Note 17.1

  • Loading and Calibration of Intracellular Ion Indicators—Note 19.1
  • MAXC Computer Program for Calculating Free Ca2+ Concentrations—Note 19.2

  • Using the Fluorescence SpectraViewer—Note 23.1
  • Selecting Optical Filters for Fluorescence Microscopy—Note 23.2
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