Click Chemistry and Other Functional Group Modifications—Chapter 3

Click Chemistry—Section 3.1

  • Principles of Bioorthogonal Labeling and Click Chemistry
  • Copper-Free Click Chemistry
  • Click-iT Azide and Alkyne Labeling Reagents
  • Click-iT Tools for Cell Proliferation Analysis
  • Click-iT TUNEL Assay
  • Click-iT Tools for Detecting RNA and Protein Synthesis
Reagents for Modifying Alcohols—Section 3.2

  • Alcohols in Proteins: Serine, Threonine and Tyrosine Residues
  • Alcohols in Carbohydrates
  • Alcohol Modification in Organic Solvents
Reagents for Modifying Aldehydes and Ketones—Section 3.3

  • Introducing Aldehydes and Ketones into Biomolecules
  • Introducing a Hydroxylamine into a Biomolecule
  • Hydrazines and Hydroxylamines
  • Aliphatic and Aromatic Amines
Derivatization Reagents for Carboxylic Acids and Carboxamides—Section 3.4

  • Coupling Hydrazines, Hydroxylamines and Amines to Carboxylic Acids
  • Esterification of Carboxylic Acids with Fluorescent Diazoalkanes
  • Fluorescent Alkyl Halides
  • Fluorescent Trifluoromethanesulfonate
  • 4-Sulfo-2,3,5,6-Tetrafluorophenol (STP) and N-Hydroxysulfosuccinimide (NHSS)