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Nucleic Acid Detection and Analysis—Chapter 8

Nucleic Acid Stains—Section 8.1

  • Properties of Cyanine Dyes
  • Premier Cyanine Dyes for Ultrasensitive Nucleic Acid Detection and Quantitation
  • Cell-Impermeant Cyanine Dimers: The TOTO Family of Dyes
  • Cell-Impermeant Cyanine Monomers: The TO-PRO Family of Dyes
  • Cell-Impermeant SYTOX Dyes for Dead-Cell Staining
  • Cell-Permeant Cyanine Dyes: The SYTO Nucleic Acid Stains
  • Chemically Reactive Cyanine Dyes
  • RediPlate 96 Nucleic Acid Stain Sampler Microplate
  • Phenanthridines and Acridines: Classic Intercalating Dyes
  • Indoles and Imidazoles: Classic Minor Groove–Binding Dyes
  • Other Nucleic Acid Stains
Labeling Oligonucleotides and Nucleic Acids—Section 8.2

  • ChromaTide Nucleotides
  • Amine-Modified Nucleotides
  • 5-Bromo-2'-Deoxyuridine, 5-Bromo-dUTP (BrdUTP) and 5-Bromo-UTP (BrUTP)
  • ULYSIS Nucleic Acid Labeling Kits
  • ARES DNA Labeling Kits
  • Labeled Oligonucleotides
  • Labeling Amine- and Thiol-Modified Oligonucleotides
  • Labeling Phosphate-Modified Oligonucleotides
  • Other Chemical Labeling Methods for Nucleic Acids
  • ElutaTube Microdialysis Vials
Nucleic Acid Quantitation in Solution—Section 8.3

  • Quant-iT Assay Kits for DNA and RNA
  • PicoGreen dsDNA Quantitation Assay
  • OliGreen ssDNA Quantitation Reagent and Kit
  • RiboGreen RNA Quantitation Assay
  • Other Stains for Nucleic Acid Quantitation in Solution
  • Real-Time Quantitative PCR Using the SYBR Green I Nucleic Acid Gel Stain
  • A Useful Technique to Reduce Background Fluorescence in Nucleic Acid Detection
Nucleic Acid Detection on Gels, Blots and Arrays—Section 8.4

  • Nucleic Acid Detection in Gels
  • Safe Imager Blue-Light Transilluminator
  • Capillary Electrophoresis and Channel Electrophoresis
Detecting Nucleic Acid Hybridization - Section 8.5

  • Principles of Fluorescence In Situ Hybridization (FISH)
  • Probe Preparation for FISH
  • Signal Amplification for FISH Using Secondary Detection Reagents
  • Counterstaining Chromosomes for FISH
  • Gaskets for In Situ Hybridization Experiments
  • Nucleic Acid Hybridization on Blots and Microarrays
  • Labeling Nucleic Acid Samples for Microarray Experiments
  • Secondary Detection for Signal Amplification
  • Tools and Techniques for Quality Control Testing and Normalization on Microarrays and Blots
  • Sample Quantitation
  • Hybridization Chambers for Microarrays
  • Solution-Based Hybridization Assays
Nuclear and Chromosome Counterstaining and Nissl Stains - Section 8.6

  • Nuclear Counterstaining of Fixed Cells and Tissues
  • Nuclear Counterstains for Live Cells and Unfixed Tissues
  • Tracking Chromosomes through Mitosis
  • Chromosome Counterstaining
  • Chromosome Banding
  • NeuroTrace Fluorescent Nissl Stains
Analysis of DNA Structure, DNA Binding and DNA Damage - Section 8.7

  • Nucleic Acid Conformational Analysis
  • Examining the Behavior of Single Nucleic Acid Molecules
  • DNA Binding Assays
  • Assessing DNA Damage
  • Assays for Enzymes that Modify Nucleic Acids