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Enzyme Substrates and Assays—Chapter 10

Introduction to Enzyme Substrates and Their Reference Standards—Section 10.1

  • Substrates Yielding Soluble Fluorescent Products
  • Substrates for Live-Cell Enzyme Assays
  • Substrates Yielding Insoluble Fluorescent or Chromophoric Products
  • Substrates Based on Excited-State Energy Transfer
  • Fluorescent Derivatization Reagents for Discontinuous Enzyme Assays
Detecting Glycosidases—Section 10.2

  • Fluorogenic β-Galactosidase Substrates
  • Modified Fluorogenic β-Galactosidase Substrates with Improved Cellular Retention
  • NovaBright β-Galactosidase Chemiluminescent Detection Kits
  • Amplex Red Galactose/Galactose Oxidase Assay Kit
  • Fluorogenic β-Glucuronidase Substrates
  • Fluorogenic β-Glucosidase Substrates
  • Amplex Red Glucose/Glucose Oxidase Assay Kit
  • Amplex Red Neuraminidase/Sialidase Assay Kit
  • EnzChek Cellulase Substrate
  • EnzChek Ultra Amylase Assay Kit
  • EnzChek Ultra Xylanase Assay Kit
  • EnzChek Lysozyme Assay Kit
  • Chromogenic Glycosidase Substrates
  • Auxiliary Products for Glycosidase Research
Detecting Enzymes That Metabolize Phosphates and Polyphosphates—Section 10.3

  • Phosphatase Substrates Yielding Soluble Fluorescent Products
  • ELF 97 Phosphate: A Phosphatase Substrate That Yields a Fluorescent Precipitate
  • BODIPY FL ATP-γ-S and BODIPY FL GTP-γ-S Thioesters: Substrates for the Fhit Nucleotide-Binding Protein
  • Chemiluminescent Phosphatase Substrates
  • Chromogenic Phosphatase Substrate
  • Kits for Detecting Phosphatases, Polymerases and Nucleases
  • Antibody Beacon Tyrosine Kinase Assay Kit
  • ATP Determination Kit
Detecting Peptidases and Proteases—Section 10.4

  • Peptidase Substrates
  • Caspase Substrates and Assay Kits
  • Substrates for HIV Protease and Renin
  • EnzChek Protease Assay Kits and Fluorescein Casein
  • EnzChek Gelatinase/Collagenase Assay Kit
  • EnzChek Elastase Assay Kit
  • DQ Substrates
  • Alternative Methods for Detecting Protease Activity
  • Protease Inhibitors
Substrates for Oxidases, Including Amplex Red Kits—Section 10.5

  • Peroxidases
  • Myeloperoxidase
  • Catalase
  • Glucose and Glucose Oxidase
  • Galactose and Galactose Oxidase
  • Cholesterol and Cholesterol Oxidase
  • Monoamine Oxidase
  • Glutamic Acid and Glutamate Oxidase
  • Acetylcholine, Acetylcholinesterase and Choline Oxidase
  • Xanthine and Xanthine Oxidase
  • Uric Acid and Uricase
Substrates for Microsomal Dealkylases, Acetyltransferases, Luciferases and Other Enzymes—Section 10.6

  • Microsomal Dealkylases
  • EnzChek Epoxide Hydrolase Substrate
  • Lipases
  • Chloramphenicol Acetyltransferase (CAT)
  • Luciferases
  • β-Lactamases
  • Dehydrogenases
  • Nitroreductase and Nitrate Reductase