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The Handbook—A Guide to Fluorescent Probes and Labeling Technologies is a comprehensive resource for fluorescence technology and its applications. Newly revised and continually updated, The Handbook contains detailed information describing the use of more than 3000 Molecular Probes products for life science research, including extensive data, numerous technical notes, and full-color images of the products in action.

Table of Contents

Introduction to Fluorescence Techniques

Part 1: Choosing a Fluorescent Label—Their Properties and Labeling Chemistries

Chapter 1
Fluorophores and Their Amine-Reactive Derivatives

Chapter 2
Thiol-Reactive Probes

Chapter 3
Reagents for Modifying Groups Other Than Thiols or Amines

Chapter 4
Biotin and Hapten Derivatives

Chapter 5
Crosslinking and Photoactivatable Reagents

Part 2: Detecting Biomolecules and Their Probes—Secondary Detection and Signal Amplification

Chapter 6
Ultrasensitive Detection Technology

Chapter 7
Antibodies, Avidins, Lectins and Related Products

Part 3: Decoding Cell Components—Nucleic Acid and Protein Analysis

Chapter 8
Nucleic Acid Detection and Analysis

Chapter 9
Protein Detection and Analysis

Chapter 10
Enzyme Substrates

Part 4: Probing Cell Structure—From Organelles and Membranes to Whole Cells

Chapter 11
Probes for Cytoskeletal Proteins

Chapter 12
Probes for Organelles