Research Highlight: The MicroArray Quality Control Project
|To help establish controls and guidelines for performing informative microarray experiments, the US Food and Drug Administration initiated the MicroArray Quality Control (MAQC) Project. A series of studies that included reagents and instruments from Ambion and Applied Biosystems, addressed the quality, reproducibility, and comparability of seven microarray platforms. Phase I results were published in the September 2006 issue of Nature Biotechnology [1-6].|
MicroArray Quality Control (MAQC) Project
The MAQC Consortium consists of scientists and statisticians from over 50 regulatory, academic, and industrial groups (including Ambion, an Applied Biosystems Business, and Applied Biosystems), who analyzed the data from more than 1,300 arrays. Their data, results, and protocols are freely available—see the sidebar, Links to Articles, Data Sets, and SOPs, to obtain this information.
Reproducibility of Gene Expression Measurements 
Validation of Microarray Results 
Microarray Performance and Normalization Techniques 
External RNA Controls 
Comparison of One- and Two-Color Microarray Platforms 
Microarray Platform Consistency in a Toxicogenomic Study 
1. Shi L, Reid LH, Jones WD, et al. (2006) The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements. Nat Biotechnol 24(9):1151–1161.
2. Canales RD, Luo Y, Willey JC, et al. (2006) Evaluation of DNA microarray results with quantitative gene expression platforms. Nat Biotechnol 24(9):1115–1122.
3. Shippy R, Fulmer-Smentek S, Jensen RV, et al. (2006) Using RNA sample titrations to assess microarray platform performance and normalization techniques. Nat Biotechnol 24(9):1123–1131.
4. Tong W, Lucas AB, Shippy R, et al. (2006) Evaluation of external RNA controls for the assessment of microarray performance. Nat Biotechnol 24(9):1132–1139.
5. Patterson TA, Lobenhofer EK, Fulmer-Smentek SB, et al. (2006) Performance comparison of one-color and two-color platforms within the MicroArray Quality Control (MAQC) project. Nat Biotechnol 24(9):1140–1150.
6. Guo L, Lobenhofer EK, Wang C, et al. (2006) Rat toxicogenomic study reveals analytical consistency across microarray platforms. Nat Biotechnol 24(9):1162–1169.