Simplified, Efficient, Single-Round RNA Amplification for Microarray Analysis
|The Ambion® MessageAmp™ Premier RNA Amplification Kit (magnetic bead RNA clean-up) is the latest innovation for the preparation of RNA samples for microarray analysis. This kit builds upon the linear RNA amplification method and reagents developed for the MessageAmp II Kits with enhancements that create: |
NEW MessageAmp™ Premier RNA Amplification Kit
The new Ambion MessageAmp Premier RNA Amplification Kit features magnetic bead-based RNA purification to yield sufficient biotinylated antisense mRNA for Affymetrix® GeneChips® from just nanogram inputs of total RNA (Figure 1). The kit incorporates extensive improvements to both workflow and sensitivity. A series of master mixes reduces the number of tubes, pipetting steps, and overall protocol steps. The result is a kit that produces enough amplified RNA (aRNA) for microarray analysis from as little as 20–25 ng of input RNA. The entire procedure from RNA amplification through hybridization to a GeneChip Array can be completed in a single day.
- Van Gelder RN, von Xastrow ME, Yool A, Dement DC, Barchas JD, Eberwine JH (1990) Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA 87:1663–1667.