All RACE- and PCR-Ready cDNA preparations synthesized with ArrayScript had a longer average cDNA length when compared to samples reverse transcribed with wild type M-MLV RT (Figure 1). Average length varied with species, tissue, and primer used. For example, average cDNA lengths were 31-93% longer when primed with oligo(dT) (Figure 1, Panel A) and 7-15% longer when primed with random decamers (Figure 1, Panel B). While these were single data points, the data represents 8 distinct samples that all show increase in length. As expected, the increase in average cDNA length is smaller for random primed products, because primers that anneal close to the termini can only produce finite-sized cDNAs.
. Longer Average cDNA Length after Reverse Transcription Using ArrayScript™. cDNAs were prepared from 2-7 µg (after adapter ligation; RACE-Ready) or 5 µg (PCR-Ready) of FirstChoice® Poly(A) RNA. The RNA was reverse transcribed using either wild type M-MLV (200 U/µl) or ArrayScript (200 U/µl), and oligo(dT) primers (A)
or random decamers (B)
. After column purification to remove primers and small reaction products, cDNA length was assessed using an Agilent® 2100 bioanalyzer. Results are expressed as average cDNA length for a given sample.