aRNA Synthesis and Labeling for Array Analysis
Amino Allyl MessageAmp™ II aRNA Amplification Kits--
Advantages of Indirect Labeling
Figure 1. aRNA Yields From Amino Allyl MessageAmp™ II aRNA Amplification Kit. The indicated amounts of Control RNA (HeLa cell total RNA) were amplified for the in vitro transcription (IVT) incubation times indicated. While longer IVT incubation times produced more aRNA, the amount of aRNA required for most microarrays was obtained with a four hour incubation with ≥500 ng of input RNA. This data was obtained using the Control RNA provided with the Amino Allyl MessageAmp II aRNA Amplification Kit. Note that aRNA yields can vary considerably with sample quality.
Figure 2. Scatter Plot Demonstrating Equivalent Labeling. Scatter plot showing signal intensities of Cy™3 vs. Cy™5 labeled aRNA synthesized with the Amino Allyl MessageAmp™ II aRNA Kit.
Now Available with Cy dyes
Improved Kit for High Yield and High Reproducibility
The Amino Allyl MessageAmp II aRNA Amplification Kit uses ArrayScript to maximize conversion of first-strand cDNA into full-length double-stranded cDNA templates. Buffers have been modified to increase labeling efficiency, and each step of the protocol has been optimized for high yields and reproducibility. Starting with 2 µg of total RNA (~60 ng mRNA), a single round of amplification using the Amino Allyl MessageAmp Kit will yield up to 150 µg aRNA. This represents a 2500-fold amplification of mRNA. In addition, as little as 10 ng total RNA (300 pg mRNA) can be amplified in a single round to produce more than 1 µg aRNA.
Amino Allyl MessageAmp is covered by patents exclusively licensed to Incyte Genomics