RNA Amplification Tips
Tip 1: Coprecipitants Improve RNA Recovery Without Affecting Amplification
Ambion offers a variety of high quality coprecipitants. These include Glycogen, GlycoBlue™ (glycogen derivatized with a blue dye to increase pellet visibility), sheared Yeast Total RNA, Yeast tRNA, and Linear Acrylamide. Each coprecipitant is subjected to stringent quality control tests to ensure that it is free of RNases as well as other unwanted contaminants.
Tip 2: Double Stranded cDNA Clean-up
Figure 1. Comparison of Two cDNA Purification Methods for Subsequent aRNA Amplification. Two identical rat thymus total RNA (2 µg) samples were amplified using the MessageAmp™ aRNA Amplification Kit. During the amplification protocol, the double stranded cDNA from one of the samples was purified using phenol:chloroform: isoamyl alcohol (pH 7.9) whereas cDNA from the other sample was subjected to Ambion's DNAclear™ (column based) clean-up protocol.