Ready for Real-time in 5 Minutes
Real-time quantitative RT-PCR (qRT-PCR) is the most sensitive technique for mRNA detection and quantitation currently available. qRT-PCR is also the preferred method for validating array analysis results, RNA interference experiments, and other techniques that evaluate gene expression changes. Ambion's Cells-to-Signal™ Kit (patent pending) is an improvement to the popular Cells-to-cDNA™ II Kit for cell lysate preparation for direct use in real-time qRT-PCR experiments. The kit uses a chemical lysis method to create cell lysates in less than 5 minutes at room temperature. The cell lysate can be used directly for RT-PCR when PCR primers are designed to span intron-exon boundaries, bypassing RNA isolation altogether. Lysates can be stored for future experiments. The Cells-to-Signal procedure is highly reproducible and is compatible with a wide variety of cell types. These improvements result in a single-step procedure that is optimized for high throughput analyses -- both for manual processing and on robotic platforms.