RETROscript® First Strand Synthesis Kit
The RETROscript® First Strand Synthesis Kit provides reagents for reverse transcription of cDNA from RNA templates. Total RNA, poly(A) RNA, tRNA, or rRNA from a variety of sources (e.g. bacterial, plant , yeast) can be used as a template for reverse transcription. With RETROscript, it is usually not necessary to isolate polyadenylated RNA, even for subsequent amplification of rare messages. Figure 1 demonstrates the amplification of 4 different RNA species from heterogeneous total RNA samples.
Figure 1. RT-PCR of Four Different RNA Species Using Decamer or Oligo dT Primers. Ambion's Total RNAs from several mouse tissues were RT-PCR amplified using Ambion's RETROscript® Kit. Random decamers or oligo dT primers, supplied in the kit, were used to prime the reverse transcription reaction. Primers specific to each of the four target messages were used in the subsequent PCR amplification. Total RNAs used were as follows: Mouse testes RNA for p53, mouse heart RNA for CAP Binding Protein, mouse liver RNA for ß-actin and S15 Ribosomal Protein.