No longer must you endure the high cost and long turn-around times required for chemically synthesized siRNA.
The new Silencer™ siRNA Construction Kit (patent pending) saves you both time and money so now you can expand your capacity for large siRNA screening experiments. More genes or more regions of the same gene can be screened for a fraction of the cost of chemically synthesized siRNA.
How the Kit Works
Construction of an siRNA begins with the acquisition of two inexpensive, desalted DNA oligonucleotides. As illustrated in Figure 1, these oligonucletides include an 8 base sequence complementary to the 3' end of the T7 promoter primer included in the Silencer™ siRNA Construction Kit. Each gene specific oligonucleotide is annealed to the supplied T7 promoter primer. A fill-in reaction with Klenow fragment generates a double-stranded template ready for use in an in vitro transcription reaction. The two transcription reaction products are hybridized to each other, treated with DNase (to remove template) and RNase (to polish the ends of the double-stranded RNA), and column purified. The entire procedure can be completed in less than 24 hours and is easily scalable -- 15 siRNA molecules can be synthesized as easily as a single siRNA. Each transcription/purification produces enough siRNA for hundreds of transfections. The time required for double-stranded RNA synthesis and purification using the Silencer™ siRNA Construction Kit is a fraction of the 12 week processing time typically required for RNA oligonucleotide synthesis via phosphoramidite chemistry and subsequent purification.
Figure 1. The Silencer™ siRNA Construction Kit Procedure.
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