End Labeling RNA for Structural Analysis
|The final folded structure of an RNA dictates its association with the cellular machinery involved in processes such as transcription, translation, and splicing, and therefore is crucial for its functional role in cellular processes. In order to more completely understand how structure controls function, RNA structural analysis is done to: |
Ambion offers two products for the preparation of high specific activity end-labeled probes for RNA structural assessment: KinaseMax™ 5' End Labeling Kit, and T4 RNA Ligase, an enzyme that can add labeled nucleotides to the 3' end of RNA. The KinaseMax Kit contains Calf Intestinal Phosphatase (CIP) and associated buffers for removal of terminal phosphates from RNA or DNA prior to labeling. A novel phosphatase removal reagent ensures elimination of CIP without organic extractions and minimizes loss of nucleic acids. The subsequent kinase reaction with T4 polynucleotide kinase in the supplied kinase buffer leads to very efficient 5' end labeling of RNA or DNA. Ambion also offers T4 RNA ligase in two sizes for 3' end labeling of the RNA.