Extraordinary Speed. Extraordinary Performance. Extraordinary Value.
Figure 1. Time Comparison of SYBR® Green-based Cells-to-CT™ Kits with Traditional Workflows Requiring RNA Purification.
Simple 7-Minute Sample Preparation: Part of a Complete Workflow
The SYBR Green Cells-to-CT Kits demonstrate performance equivalent to that of purified RNA, as shown by by high correlation of real-time PCR data obtained from both methods (Figure 3). All the required reagents are included in the kits, with the exception of PCR primer sets specific for your targets of interest.
Figure 3. Power SYBR® Green and Fast SYBR® Green Cells-to-CT™ Kits Perform Equivalently to Purified RNA Over a Broad Set of Gene Targets. A cell mixture (10,000 cells) comprised of HeLa, HegG2, Jurkat, HEK-293, and U-87-MG cells were analyzed using either traditional RNA purification and real-time PCR, or the Power SYBR Green and Fast SYBR Green Cells-to-CT Kits. Technical (real-time PCR) quadruplicates were performed for each method for each of the 155 primer sets. A high correlation was observed between the traditional purified RNA real-time PCR results and results obtained using the Power SYBR Green Cells-to-CT Kit (y = 0.94x + 1.46, R2= 0.96) and Fast SYBR Green Cells-to-CT Kit (y = 0.97x +0.45, R2=0.96).
Achieve Superior Sensitivity and Dynamic Range
Figure 4. Sensitivity of the Power and Fast SYBR® Green Cells-to-CT™ Kits Versus Competing Technologies. HeLa cells (100–100,000) were prepared using either traditional RNA isolation, the Power or Fast SYBR® Green Cells-to-CT™ Kit protocols, or other lysate methods from Competitors I and Q. Real-time PCR was performed in triplicate using a primer set for PPIA.
Make it Even Faster with Fast SYBR Green Master Mix
Whichever kit you choose, the new Power SYBR Green and Fast SYBR Green Cells-to-CT Kits provide quick, easy, and complete solutions for real-time RT-PCR from cells.
Nathan Harris, Annmarie Schramm, Richard Fekete, Annie Nguyen • Applied Biosystems Inc., Austin, TX