Ambion’s magnetic bead-based MagMAX™ Viral RNA Isolation Kit
Ambion’s magnetic bead-based MagMAX™ Viral RNA Isolation Kit is optimized for isolating viral RNA from biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. RNA isolation from cell-free samples presents some unique challenges compared to RNA isolation from tissue samples or cultured cells. These include low viral titers, large volumes, and complex samples—which often contain extracellular proteins and high levels of RNase. Since researchers and diagnosticians often need to conduct large scale screening experiments for large studies or during viral outbreaks, the process must also be rapid and reproducible, as well as easily adapted for both manual and robotic high throughput processing. Finally, the procedure must be compatible with real-time qRT-PCR, the standard for diagnostic detection of viral RNA. The MagMAX Viral RNA Isolation Kit addresses these issues. It offers the advantages of solid phase extraction using glass fiber filters—it is fast and requires no organic solvents or RNA precipitation while eliminating the problems often encountered with filter-based methods, such as clogging, large elution volumes, and inconsistent RNA yield. MagMAX Kits are available for both manual and high throughput processing.
Get Better Viral RNA Detection from 10-fold Less Sample
Figure 1. MagMAX™ Achieves Better Sensitivity with 10X Less Sample than Competitor’s Kit. The sensitivity of viral RNA detection using Ambion’s MagMax Kit was compared with that of the current NVSL-validated viral testing protocol (competitor). Clinical tracheal and cloacal swab samples were collected from poultry infected with California exotic newcastle disease (END) virus. RNA was isolated from 50 µl of sample using the MagMAX protocol or from 500 µl of sample using a competitor's product. Even with 10-fold less sample, viral RNA detection in RNA isolated with the MagMAX protocol was as sensitive or more sensitive than from RNA isolated using the competitor’s kit.
Advantages of Magnetic Beads for Viral RNA Isolation
A Fast and Simple Processing Method
Recover a Broad Size Range of RNA from Diverse Samples
Linear Recovery Down to 50 Copies of Transcript
Figure 3. Linear Recovery of Synthetic Viral RNA from Biological Fluids. MagMAX™ Lysis Buffer was added to samples of water, plasma, serum, and milk, and the mixture was then spiked with a serial dilution of a 466 nt HIV Armored RNA® transcript. (Armored RNA is protected from nucleases by a protein coat derived from the MS2 coat protein gene.) Viral RNA was isolated using the MagMAX™-96 Viral RNA Isolation Kit. The recovered RNA was analyzed by qRT-PCR. The results demonstrate that as few as 50 input copies of transcript can be successfully detected in RNA isolated using the MagMAX Kit.