The MELT system is now available with the option to isolate genomic DNA from solid tissues, including mouse tails. Although conventional mouse tail DNA isolation procedures require lengthy, overnight digestions, MELT provides both tissue digestion and DNA isolation protocols that can be completed in just hours, enabling same day results. DNA can be purified from MELT lysates (Figure 2) with reagents provided in the current kit format, along with RNase A (not included) for an optional RNase treatment step. The purified DNA is suitable for both endpoint and real-time PCR.
DNA Isolation Using the MELT™ Total Nucleic Acid Isolation System. (A) 1% Agarose gel, ethidium bromide stained, displays DNA isolated from various tissue types with the MELT System (~500 ng of each sample per lane). (B) Real-time PCR targeting GAPDH and FAS genomic sequences using 100 ng input RNA and TaqMan® technology (ABI). Error bars indicate the range from duplicate reactions.