RiboPure™ Technology - For Tissue, Cells, Blood, Bacteria and Yeast
Researchers performing array analysis have found that a combination of phenol based and solid phase RNA extraction methods often provide superior array data than either method alone (1-3). Ambion's new line of RiboPure RNA Isolation Kits are the first kits that combine these RNA extraction methods. Four different kits are now available for the isolation of high quality total RNA from a variety of samples:
High Quality RNA Isolation
Fast Results and High Yields with Small Samples
New! Isolate High Quality Total RNA from Yeast
Figure 1. Yeast Total RNA Isolated Using the RiboPure-Yeast Kit. P. pastoris, S. cerevisiae RNA, C. albicans, and S. pombe RNA, isolated using the RiboPure-Yeast Kit and treated with DNase, was run on an Agilent bioanalyzer chip. The ratio of 28S:18S rRNA is given for each sample.
Isolate High Quality Total RNA from Bacteria
Figure 3. High Quality Bacterial RNA Obtained with RiboPure-Bacteria Kit. Total RNA (2 µg) was separated on a 1% agarose gel in formaldehyde/MOPS buffer. Samples were heated at 80ºC for 10 min in denaturing agarose gel loading buffer with 100 µg/µl EtBr before electrophoresis at 5 V/cm
RiboPure-WBC: Scalable Protocol for Isolating High Quality RNA from Blood
The RiboPure-WBC Kit protocol consists of three steps: 1) fractionation of whole blood by centrifugation, recovery of the WBCs and RNA stabilization in Ambion's RNAlater® (provided), 2) lysis of WBCs in guanidinium and initial RNA purification by phenol/chloroform extraction, and 3) final RNA purification on a glass fiber filter.
The kit provides sufficient reagents for the purification and stabilization of WBCs from 40 whole blood samples of up to 10 ml each (2.5-10 ml of whole blood can be used per isolation). The RNA purified with the RiboPure-WBC Kit can be used in the most demanding downstream applications, such as real-time RT-PCR or array analysis. Average yields of RNA range between 4-20 µg from 5 ml of whole blood; the variation is due to differences in WBC numbers between samples.