Faster Nucleic Acid Isolation From FFPE Tissues
|Applied Biosystems has improved the protocol for the Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE Tissues, resulting in significantly reduced processing time. No reagents or formulations have changed—only pipetting volumes, incubation times, and temperatures. The end result is a shortened protocol with equivalent nucleic acid yield and performance to that of the original protocol.|
FFPE Tissues Abundant but Challenging
A Faster Isolation Workflow
Figure 1. Faster RecoverAll™ Total Nucleic Acid Isolation Kit Workflow.
Faster Recovery Without Sacrificing Results
Figure 2. New, Faster RecoverAll™ Isolation Kit Protocol Gives RNA with Better Real-time PCR Performance than Original Protocol. Real-time RT-PCR was carried out for 4 targets (GAPDH, GUSB, miR-16 and miR-155) with RNA isolated from breast, lung and colon FFPE tissue using the new RecoverAll Isolation Kit protocol or the original protocol. In all cases, RNA isolated with the new protocol shows equivalent or lower CT values (better performance) than RNA isolated with the original protocol.
RecoverAll Kit Outperforms the Competition
In addition, the Ambion RecoverAll Kit takes exactly 17 steps to complete the isolation of RNA from FFPE tissue samples with an overall RNA recovery time of 1–1.5 hr.
DNA Isolation with the RecoverAll Kit
Emily Zeringer, Tim Barta, Rick Conrad • Applied Biosystems