Which Water to Use? Avoid RNase contamination in reagents
by Sapna Chacko
Ambion Certified and Guaranteed Nuclease-free
- DEPC-treated Water - DEPC destroys enzymatic activity by modifying -NH2, -SH and -OH groups in RNases and other proteins. DEPC treatment is a very effective way to treat solutions that will contact RNA. Ambion's DEPC-treated water is autoclaved both before and after packaging to ensure sterility and complete inactivation of DEPC.
- Nuclease-free Water (not DEPC-treated) - Ambion also offers nuclease-free water that is not DEPC-treated. Ambion's Nuclease-free Water is ideal for applications that are reported to be acutely sensitive to residual DEPC e.g., oocyte injection and other in vivo translation applications.
- RT-PCR Grade Water - Ambion's RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using a sensitive end-point PCR assay based on detection of rRNA genes. Prokaryotic DNA contamination is assessed by performing PCR with 16S rRNA primers, and eukaryotic DNA contamination is assessed by performing PCR with 18S rRNA primers. Each lot is also tested for nuclease contamination. This product is ideal for use in any PCR or RT-PCR application.
Bulk and Custom Orders
Inactivate RNases in Solution
The RNAsecure™ Reagent eliminates these problems. RNAsecure is a unique nonenzymatic reagent that will irreversibly inactivate RNases in solution. Unlike DEPC, RNAsecure can be used with virtually any solution and does not require post-treatment autoclaving. The inactivation process can be repeated at any time to protect against introduced contaminants. RNAsecure does not interfere with downstream procedures, such as RT-PCR and in vitro transcription, and is also compatible with Tris and other solutions that cannot be treated with DEPC.
The RNAsecure™ Resuspension Solution contains the same active ingredients as the RNAsecure Reagent, but is supplied at working concentration for direct resuspension of RNA pellets.