Is Your DNase RNase-Free?
|By Gary Latham and Meenal Patel, R&D Scientists at Ambion |
Recently, Ambion introduced the RNase Alert¹ Kit. This simple, single-step assay tests solutions for contamination by a wide variety of RNases in only 30 minutes. RNase activity is visualized on a transilluminator or fluorometer.
Figure 1. RNaseAlert¹ Substrate Cleavage with RNase A. Relative fluorescence units (RFU) generatd by digesting the RNaseAlert¹ substrate with 0.5 pg, 5 pg and 50 pg of RNase A. The substrate contains a fluorophore and a quencher and will fluoresce when cleaved by RNases.
Figure 2. RNaseAlert¹ Substrate Cleavage with Supplier 'S' DNase I. Relative fluorescence units (RFU) generated during incubation of the RNaseAlert¹ substrate with DNase I from supplier 'S'.
Figure 3. RNaseAlert¹ Substrate Cleavage with Ambion DNase I. Relative fluorescence units (RFU) generated during incubation of RNaseAlert¹ substrate with Ambion's DNase I. Lack of fluorescence indicates no RNase present in the sample.