Stable Markers for Northern Analysis
|Ambion's Millennium Markers™ are designed to provide accurate size determination of single-stranded RNA transcripts from 0.5-9 kb and can be used in any Northern protocol. Because of their even spacing, Millennium Markers are ideal for constructing very accurate standard curves for mRNA size determination. They also provide a reference for easy verification and comparison of known bands. |
RNA markers are more stable (Figure 1) and resistant to RNases (Figure 2) when stored in formamide. Therefore, we now provide the Millennium Markers in formamide. To use, simply add the Northern Loading Buffer to the markers and heat, just as you would treat your RNA samples.