Premade, RNase-free Northern Reagents
Northern analysis remains a standard method for the detection and quantitation of mRNA despite the advent of more sensitive techniques such as RT-PCR. In addition to being a quantitative tool, Northern analysis has the advantage of being the only method able to simultaneously provide information about the size, alternative splicing and integrity of RNA samples. However, inefficiencies in any of the several steps in the procedure, or ribonuclease contamination can lead to a loss in the sensitivity of Northern analysis. Ambion's Northern Max™ Kits and reagents eliminate these procedural inefficiencies, and are designed to maximize sensitivity.
Complete Northern Systems
RNase-Free Reagents for Northerns
NorthernMax reagents are the same components included in the NorthernMax Kits, but in larger sizes. All NorthernMax reagents are supplied ready-to-use and are certified RNase-free. Comprehensive instructions for the use of these products are included.
Figure 1. ULTRAhyb™ Detects a Signal in Hours; Other Hybridization Solutions Require Days. Duplicate Northern blots were prepared using mouse thymus total RNA. Each blot was hybridized with DNA probe to p53 at a concentration of 1 x 106 cpm probe/ml hybridization solution. Incubation and washing conditions were as per protocol for each solution. Blots were exposed to film for the indicated times. Current Protocols in Molecular Biology, 1994. Ausubel, F.M. and others, Editors John Whiley & Sons, Inc. Molecular Cloning: A Laboratory Manual, 1989. Sambrook, J. Fritsch, E.F., Maniatis, T. Cold Spring Harbor Laboratory Press